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An In Vivo Mouse Model to Study Blood–Brain Barrier Destabilization in the Chronic Phase of Stroke

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The Blood-Brain Barrier

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2492))

Abstract

Cerebral ischemic injury evokes a complex cascade of pathophysiological events at the blood–vascular–parenchymal interface. These evolve over time and space and result in progressive neurological damage. Emerging evidence suggests that blood–brain barrier (BBB) recovery and reestablishment of BBB impermeability are incomplete and that these could influence stroke injury recovery, increase the risk of new stroke occurrence, and be a solid substrate for developing vascular dementia. Recent work from the author’s laboratory has established the existence of incomplete BBB recovery in chronic stroke conditions that was induced by structural alterations to brain endothelial junctional complexes and persistent BBB leakage. The experimental methodology presented here is focused on modelling chronic stroke injury using an in vivo thromboembolic mouse stroke model and how to evaluate the kinetics and magnitude of BBB hyperpermeability in chronic stroke conditions using a combination of magnetic resonance imaging, tracer studies, and immunohistochemistry.

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Acknowledgments

This work is supported by NIH grants: AG057928 to A.V.A. and NS098211 to S.M.S.

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Authors and Affiliations

  1. Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA

    Svetlana M. Stamatovic & Anuska V. Andjelkovic

  2. Graduate Program in Neuroscience, University of Michigan Medical School, Ann Arbor, MI, USA

    Chelsea M. Phillips

  3. Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI, USA

    Richard F. Keep & Anuska V. Andjelkovic

  4. Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI, USA

    Richard F. Keep

Authors
  1. Svetlana M. Stamatovic
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  2. Chelsea M. Phillips
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  3. Richard F. Keep
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  4. Anuska V. Andjelkovic
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Corresponding author

Correspondence to Anuska V. Andjelkovic .

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Editors and Affiliations

  1. Discovery Pharmacology, COMPASS Pathways PLC, London, UK

    Nicole Stone

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Stamatovic, S.M., Phillips, C.M., Keep, R.F., Andjelkovic, A.V. (2022). An In Vivo Mouse Model to Study Blood–Brain Barrier Destabilization in the Chronic Phase of Stroke. In: Stone, N. (eds) The Blood-Brain Barrier. Methods in Molecular Biology, vol 2492. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2289-6_17

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  • DOI: https://doi.org/10.1007/978-1-0716-2289-6_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2288-9

  • Online ISBN: 978-1-0716-2289-6

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