Abstract
Avian influenza virus and some mammalian influenza A viruses are usually isolated, propagated, and titrated in embryonated chicken eggs (ECE). Most any sample type can be accommodated for culture with appropriate processing. Isolation may also be accomplished in cell culture particularly if mammalian lineage isolates are suspected, for example, swine influenza in turkey specimens. Culture is highly sensitive, but is not specific for influenza A, which may be an advantage because a sample may be screened for several agents at once. Once an agent is isolated in culture, the presence of influenza viruses is confirmed with any of several assays. The methods for propagating virus isolates in ECE are described.
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Spackman, E., Killian, M.L. (2014). Avian Influenza Virus Isolation, Propagation, and Titration in Embryonated Chicken Eggs. In: Spackman, E. (eds) Animal Influenza Virus. Methods in Molecular Biology, vol 1161. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0758-8_12
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DOI: https://doi.org/10.1007/978-1-4939-0758-8_12
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-0758-8
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