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Dopamine Receptors in the Subthalamic Nucleus: Identification and Localization of D5 Receptors

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Book cover Dopamine Receptor Technologies

Part of the book series: Neuromethods ((NM,volume 96))

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Abstract

Herein we present methodological approaches for the identification and characterization of dopamine receptors in the subthalamic nucleus, a component nucleus of the basal ganglia, at pre-and postsynaptic locations and of their roles with an emphasis given to the dopamine D5 receptor subtype. This chapter focuses on the possible sources of divergence between electrophysiological studies and describes the pharmacological tools available for functional studies of this receptor. The procedures for single-cell reverse transcription PCR (polymerase chain reaction) identification of dopamine D5 receptor mRNA and the immunochemical detection of the receptor at cellular and subcellular levels are presented.

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Acknowledgments

We wish to thank the Regional Council of Aquitaine and Fondation de France who partly supported this work by grants 12006005 (FCAN scheme), and 2005013850 and 00016810, respectively. Financial support was also provided by CNRS and University of Bordeaux. D. Sibley (NINDS, Bethesda) and J. Drago (Monash University) allowed us to use the D5R and D1R mutant mice they engineered. J. Waddington (Royal College of Surgeons, Dublin) donated the D5R mutant mice we used to generate a colony. L.F received a PhD fellowship from MRT.

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Authors and Affiliations

  1. Univ. Bordeaux, Institut des Maladies Neurodégénératives, UMR 5293, 33000, Bordeaux, France

    Lionel Froux, Jerome Baufreton & Anne Taupignon

  2. CNRS, Institut des Maladies Neurodégénératives, UMR 5293, 33000, Bordeaux, France

    Lionel Froux, Jerome Baufreton & Anne Taupignon

  3. Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Ciencias, 29071, Málaga, Spain

    Diana Suarez-Boomgaard & Alicia Rivera

  4. Univ. Bordeaux, INCIA, UMR 5287, 33000, Bordeaux, France

    Maurice Garret

  5. CNRS, INCIA, UMR 5287, 33000, Bordeaux, France

    Maurice Garret

  6. IMN, Université de Bordeaux, 146 rue Saignat, 33076, Bordeaux, France

    Anne Taupignon

Authors
  1. Lionel Froux
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  2. Diana Suarez-Boomgaard
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  3. Jerome Baufreton
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  4. Alicia Rivera
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  5. Maurice Garret
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  6. Anne Taupignon
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Corresponding author

Correspondence to Anne Taupignon .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute and University of Ottawa, Ottawa, Ontario, Canada

    Mario Tiberi

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Froux, L., Suarez-Boomgaard, D., Baufreton, J., Rivera, A., Garret, M., Taupignon, A. (2015). Dopamine Receptors in the Subthalamic Nucleus: Identification and Localization of D5 Receptors. In: Tiberi, M. (eds) Dopamine Receptor Technologies. Neuromethods, vol 96. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2196-6_9

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  • DOI: https://doi.org/10.1007/978-1-4939-2196-6_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2195-9

  • Online ISBN: 978-1-4939-2196-6

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