Abstract
Neuronal communication requires a constant adjustment of the number and subtype of neurotransmitter receptors and ion channels at the plasma membrane. Although classical fluorescence ensemble approaches have provided valuable insights into cellular and molecular pathways, their inherent limitations (e.g., average behaviors, spatial resolution) have prompted neuroscientists to use and adapt other imaging approaches. Among these, single nanoparticle tracking offers a remarkable mean to explore, at the single neurotransmitter receptor and ion channel level, the behavior of these molecular actors in living brain cells. In this chapter, we describe the procedure and experimental steps necessary to perform single nanoparticle tracking of membrane neurotransmitter receptors and ion channels in living brain cells.
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Acknowledgments
This work was supported by the Centre National de la Recherche Scientifique, Agence Nationale de la Recherche, Conseil Régional d’Aquitaine, and Marie Curie Individual Fellowship 326442. We thank lab members for constructive discussions and critical reading of the manuscript and the Bordeaux Imaging Center for technical support.
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Varela, J., Dupuis, J., Groc, L. (2016). Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells. In: Luján, R., Ciruela, F. (eds) Receptor and Ion Channel Detection in the Brain. Neuromethods, vol 110. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3064-7_5
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DOI: https://doi.org/10.1007/978-1-4939-3064-7_5
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3063-0
Online ISBN: 978-1-4939-3064-7
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