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Cell Migration pp 291–311Cite as

Using Single-Protein Tracking to Study Cell Migration

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1749))

Abstract

To get a complete understanding of cell migration, it is critical to study its orchestration at the molecular level. Since the recent developments in single-molecule imaging, it is now possible to study molecular phenomena at the single-molecule level inside living cells. In this chapter, we describe how such approaches have been and can be used to decipher molecular mechanisms involved in cell migration.

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Abbreviations

AOTF:

Acousto-optic tunable filter

Cas9:

CRISPR associated protein 9

CRISPR:

Clustered regularly interspaced short palindromic repeats

D :

Diffusion coefficient

DMEM:

Dulbecco’s modified Eagle medium, high glucose

EDTA:

Ethylenediaminetetraacetic acid

EMCCD:

Electron-multiplying charge-coupled device

FBS:

Fetal bovine serum

FWHM :

Full width at half maximum

GFP:

Green fluorescent protein

MEF:

Mouse embryonic fibroblast

mEos2:

Monomeric Eos2

MSD:

Mean square displacement

NA:

Numerical aperture

PALM:

Photoactivation localization microscopy

PBS:

Phosphate buffered saline

sCMOS:

Scientific complementary metal-oxide semiconductor

SRR:

Superresolved reconstruction image

STL:

Superresolution time-lapse movie

TIRF:

Total internal reflection fluorescence

uPAINT:

Universal point accumulation imaging in nanoscale topography

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Author notes

  1. Thomas Orré and Amine Mehidi contributed equally to this work.

Authors and Affiliations

  1. Interdisciplinary Institute for Neuroscience, UMR 5297, University of Bordeaux, Bordeaux, France

    Thomas Orré, Amine Mehidi, Sophie Massou, Olivier Rossier & Grégory Giannone

  2. Interdisciplinary Institute for Neuroscience, UMR 5297, CNRS, Bordeaux, France

    Thomas Orré, Amine Mehidi, Sophie Massou, Olivier Rossier & Grégory Giannone

Authors
  1. Thomas Orré
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  2. Amine Mehidi
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  3. Sophie Massou
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  4. Olivier Rossier
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  5. Grégory Giannone
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Corresponding author

Correspondence to Grégory Giannone .

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Editors and Affiliations

  1. Laboratory of Biochemistry, Ecole Polytechnique, Palaiseau, France

    Alexis Gautreau

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Orré, T., Mehidi, A., Massou, S., Rossier, O., Giannone, G. (2018). Using Single-Protein Tracking to Study Cell Migration. In: Gautreau, A. (eds) Cell Migration. Methods in Molecular Biology, vol 1749. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7701-7_21

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