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Purinergic Signaling pp 243–259Cite as

P2X Electrophysiology and Surface Trafficking in Xenopus Oocytes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2041))

Abstract

Xenopus oocytes serve as a standard heterologous expression system for the study of various ligand-gated ion channels including ATP P2X receptors. Here we describe the whole-cell two-electrode voltage clamp and biotinylation/Western blotting techniques to investigate the functional properties and surface trafficking from P2X-expressing oocytes.

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Authors and Affiliations

  1. Institut des Maladies Neurodégénératives, CNRS UMR 5293, Université de Bordeaux, Bordeaux, France

    Eléonore Bertin, Audrey Martínez & Eric Boué-Grabot

Authors
  1. Eléonore Bertin
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  2. Audrey Martínez
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  3. Eric Boué-Grabot
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Corresponding author

Correspondence to Eric Boué-Grabot .

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Editors and Affiliations

  1. BioMedical Research Institute of Murcia (IMIB), Clinical University Hospital Virgen de la Arrixaca, Murcia, Spain

    Pablo Pelegrín

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Bertin, E., Martínez, A., Boué-Grabot, E. (2020). P2X Electrophysiology and Surface Trafficking in Xenopus Oocytes. In: Pelegrín, P. (eds) Purinergic Signaling. Methods in Molecular Biology, vol 2041. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9717-6_18

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  • DOI: https://doi.org/10.1007/978-1-4939-9717-6_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9716-9

  • Online ISBN: 978-1-4939-9717-6

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