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Applications of MicroArrays for Mass Spectrometry (MAMS) in Single-Cell Metabolomics

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Single Cell Metabolism

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2064))

Abstract

The metabolic network is the endpoint in the flow of information that begins with the “gene” and ends with “phenotype” (observable function) of the cell. Previously, due to the variety of metabolites analyzed inside cells, the metabolomic measurements were performed with samples including multiple cells. Unfortunately, this sampling process may mask important metabolic phenomena, such as cell-to-cell heterogeneity. For these studies, we must use analytical techniques that can robustly deliver reproducible results with single-cell sensitivity. In this chapter, we summarize laser-based methods for single-cell analysis and a novel approach of MicroArrays for Mass Spectrometry (or MAMS) is described in full detail. This particular type of microarrays was tailored for the study of cells grown in liquid medium using multiple-analytical read-outs, such as optical and laser desorption/ionization (LDI) or MALDI mass spectrometry.

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Author information

Authors and Affiliations

  1. Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland

    Alfredo J. Ibáñez

  2. Zurich PhD Program Molecular Life Sciences, Life Science Zurich, Zurich, Switzerland

    Alfredo J. Ibáñez

  3. Instituto de Ciencias Ómicas y Biotecnología Aplicada, Pontificia Universidad Católica del Perú, Lima, Peru

    Alfredo J. Ibáñez

  4. Research Group Mass Spectrometry, Max Planck Institute for Chemical Ecology, Jena, Germany

    Ales Svatos

Authors
  1. Alfredo J. Ibáñez
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  2. Ales Svatos
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Corresponding author

Correspondence to Ales Svatos .

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Editors and Affiliations

  1. Waters Corporation, Milford, MA, USA

    Bindesh Shrestha

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Ibáñez, A.J., Svatos, A. (2020). Applications of MicroArrays for Mass Spectrometry (MAMS) in Single-Cell Metabolomics. In: Shrestha, B. (eds) Single Cell Metabolism. Methods in Molecular Biology, vol 2064. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9831-9_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9831-9_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9829-6

  • Online ISBN: 978-1-4939-9831-9

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