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Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes

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Protein Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 869))

Abstract

The two-dimensional blue native/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D BN/SDS-PAGE) is a method of choice for the investigation of protein complexes. This highly resolvent separation method is unique in that it facilitates the identification of many protein complexes simultaneously. Because of its simplicity and suitability, the 2D BN/SDS-PAGE can be now applied to a wide range of organisms, including bacteria, viruses, yeasts, animals, and plants. Moreover, recent modifications have made it possible to apply this method to the study of whole protein complexes of an organism. Here, we propose protocols for the investigation of the whole complexome of bacteria as well as eukaryotic cells.

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Acknowledgments

Particular thanks are extended to Prof. Marc Bonneu for his innovative and brilliant ideas. The study on Helicobacter pylori was financially supported by INSERM, the University of Bordeaux 2, the European consortium INCA (Infection and Cancer) from the sixth PCRD of the EU, the Conseil Régional d’Aquitaine, and the Association pour la Recherche sur le Cancer (ARC). The study on Escherichia coli was financially supported by the University of Bordeaux 2 and the Conseil Régional d’Aquitaine. The study on Yarrowia lipolytica was financially supported by INRA and CNRS and Oxyrane (grant no. B 05344). Predoctoral and postdoctoral students were supported in part by fellowships from the Fondation pour la Recherche Médicale (H. pylori), by EU grant number QLRICT-2001-00015 under the RTD program “Quality of Life and Management of Living Resources” (E. coli). We thank Lindsay Megraud for proofreading the manuscript.

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Authors and Affiliations

  1. Laboratoire de Bactériologie, INSERM U853, Université Victor Segalen Bordeaux 2, Bordeaux Cedex, France

    Jean Paul Lasserre & Armelle Ménard

  2. Pôle Protéomique, Plateforme Génomique Fonctionnelle, Université Victor Segalen Bordeaux 2, Bordeaux, France

    Jean Paul Lasserre

  3. Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, Université Victor Segalen/Bordeaux II, Bordeaux Cedex, France

    Jean Paul Lasserre

Authors
  1. Jean Paul Lasserre
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  2. Armelle Ménard
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Correspondence to Armelle Ménard .

Editor information

Editors and Affiliations

  1. Oklahoma Medical Research Foundation, Univ. of Oklahoma Health Sciences Center, NE. 13th Street 825, Oklahoma City, 73104, Oklahoma, USA

    Biji T. Kurien

  2. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, Oklahoma, USA

    R. Hal Scofield

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Lasserre, J.P., Ménard, A. (2012). Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_27

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  • DOI: https://doi.org/10.1007/978-1-61779-821-4_27

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-820-7

  • Online ISBN: 978-1-61779-821-4

  • eBook Packages: Springer Protocols

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