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In Utero Electroporation to Study Mouse Brain Development

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Brain Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1082))

Abstract

In utero electroporation is a rapid and powerful technique to study the development of many brain regions. This approach presents several advantages over other methods to study specific steps of brain development in vivo, from proliferation to synaptic integration. Here, we describe in detail the individual steps necessary to carry out the technique. We also highlight the variations that can be implemented to target different cerebral structures and to study specific steps of development.

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Authors and Affiliations

  1. Neurocentre Magendie, Physiopathologie de la Plasticité Neuronale, U862, Institut National de la Santé et de la Recherche Médicale, Université de Bordeaux, Bordeaux, France

    Emilie Pacary

  2. Division of Molecular Neurobiology, MRC National Institute for Medical Research, London, UK

    François Guillemot

Authors
  1. Emilie Pacary
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  2. François Guillemot
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Editors and Affiliations

  1. Department of Biology, University of Fribourg, Fribourg, Switzerland

    Simon G Sprecher

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Pacary, E., Guillemot, F. (2014). In Utero Electroporation to Study Mouse Brain Development. In: Sprecher, S. (eds) Brain Development. Methods in Molecular Biology, vol 1082. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-655-9_19

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  • DOI: https://doi.org/10.1007/978-1-62703-655-9_19

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-654-2

  • Online ISBN: 978-1-62703-655-9

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