Skip to main content
SpringerLink
Log in

Evidence that 5-HT1A receptors are involved in the adrenaline-releasing effects of 8-OH-DPAT in the conscious rat

  • Short Communications
  • Published:
Naunyn-Schmiedeberg's Archives of Pharmacology Aims and scope Submit manuscript

Summary

8-Hydroxy-2-(di-n-propylamino)tetralin (8OH-DPAT) is a 5-HT1A receptor-selective agonist that has recently been reported to trigger adrenal catecholamine release and hyperglycemia. The aim of this study was to analyze in the conscious rat whether the 5-HT1A receptor subtype is involved in these effects.

8-OH-DPAT (0.1–1 mg/kg, i.v.) evoked dose-dependent increases in plasma adrenaline and glucose concentrations. Increases in plasma adrenaline levels peaked 5 min after administration of 8-OH-DPAT; in contrast, plasma glucose levels rose throughout the 20 min period of analysis. Prior administration of (−)pindolol, a beta-adrenoceptor antagonist that blocks 5-HT1A receptors, markedly diminished the rise in plasma adrenaline levels and abolished the hyperglycemia triggered by 8-OHD-PAT. On the other hand, neither the selective beta 1-adrenoceptor antagonist, betaxolol, the selective beta 2-adrenoceptor antagonist, ICI 118.551, nor the 5-HT2 receptor antagonist, ketanserin, affected 8-OH-DPAT-induced increases in plasma adrenaline levels. In addition, except for ICI 118.551 pretreatment, which delayed the hyperglycemic effect of 8-OH-DPAT, none of these antagonists affected the rise in glycaemia evoked by 8-OHD-PAT.

The data suggest that the adrenaline-releasing and a major part of the hyperglycemic effects of 8-OH-DPAT are mediated by activation of 5-HT1A receptors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anton AH, Sayre DF (1962) A study of the factors affecting the aluminum oxide-trihydroxyindole procedure for the analysis of catecholamines. J Pharmacol Exp Ther 138:360–375

    Google Scholar 

  • Bagdy G, Szemeredi K, Murphy DL (1989) Marked increases in plasma catecholamine concentrations precede hypotension and bradycardia caused by 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) in conscious rats. J Pharm Pharmacol 41:270–272

    Google Scholar 

  • Berthoud HR, Trimble ER, Siegel EG, Bereiter DA, Jeanrenaud B (1980) Cephalic-phase insulin secretion in normal and pancreatic islet-transplanted rats. Am J Physiol 238:E336-E340

    Google Scholar 

  • Chaouloff F, Jeanrenaud B (1987) 5-HT1A and alpha 2-adrenergic receptors mediate the hyperglycemic and hypoinsulinemic effects of 8-hydroxy-2-(di-n-propylamino)tetralin in the conscious rat. J Pharmacol Exp Ther 243:1159–1166

    Google Scholar 

  • Chaouloff F, Baudrie V, Laude D (1990) Evidence that the 5-HT1A receptor agonists buspirone and ipsapirone activate adrenaline release in the conscious rat. Eur J Pharmacol (in press)

  • Gilbert F, Brazell C, Tricklebank MD, Stahl SM (1988) Activation of the 5-HT1A receptor subtype increases plasma ACTH concentration. Eur J Pharmacol 147:431–439

    Google Scholar 

  • Hamon M, Bourgoin S, Gozlan H, Hall MD, Goetz C, Artaud F, Horn AS (1984) Biochemical evidence for the 5-HT agonist properties of PAT [8-hydroxy-2-(di-n-propylamino)tetralin] in the rat brain. Eur J Pharmacol 100:263–276

    Google Scholar 

  • Hoyer D (1988) Functional correlates of serotonin 5-HT1 recognition sites. J Receptor Res 8:59–81

    Google Scholar 

  • Koenig JI, Gudelsky GA, Meltzer HY (1987) Stimulation of corticosterone and B-endorphin secretion in the rat by selective 5-HT receptor subtype activation. Eur J Pharmacol 137:1–8

    Google Scholar 

  • Lum BKB, Lau YS, Buesa R, Lockwood R, Kuo SH (1980) Studies on the hyperglycaemia and hepatic glycogenolysis produced by alpha and beta adrenergic agonists in the cat. Life Sci 26:1195–1202

    Google Scholar 

  • Middlemiss DN, Fozard JR (1983) 8-Hydroxy-2-(di-n-propylamino)tetralin discriminates between subtypes of the 5-HT1 recognition site. Eur J Pharmacol 90:151–153

    Google Scholar 

  • Nahorski SR, Willcocks AL (1983) Interactions of B-adrenoceptor antagonists with 5-hydroxytryptamine receptor subtypes in rat cerebral cortex. Br J Pharmacol 78:107P

    Google Scholar 

  • Peroutka SJ (1985) Selective interaction of novel anxiolytics with 5-hydroxytryptamine1A receptors. Biol Psychiatry 20:971–979

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Pharmacologie, Groupe de Neuropharmacologie, INSERM U7, CHU Necker-E. M., 156 rue de Vaugirard, 75015, Paris, France

    F. Chaouloff, V. Baudrie & D. Laude

Authors
  1. F. Chaouloff
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. Baudrie
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Laude
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Send offprint requests to F. Chaouloff at the above address

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chaouloff, F., Baudrie, V. & Laude, D. Evidence that 5-HT1A receptors are involved in the adrenaline-releasing effects of 8-OH-DPAT in the conscious rat. Naunyn-Schmiedeberg's Arch Pharmacol 341, 381–384 (1990). https://doi.org/10.1007/BF00180665

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00180665

Key words

Search

Navigation