Skip to main content
Log in

Immunocytochemical differentiation between adipokinetic hormone (AKH)-like peptides in neurons and glandular cells in the corpus cardiacum of Locusta migratoria and Periplaneta americana with C-terminal and N-terminal specific antisera to AKH

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

An immunocytochemical method was used to differentiate between immunoreactive substances in glandular cells in the corpora cardiaca (CC) and in certain cerebral neurons in 2 insect species, Locusta migratoria migratorioides and Periplaneta americana. The staining properties of antisera raised to different parts of the decapeptide adipokinetic hormone (AKH) were compared and their specificity was determined by preabsorption with AKH and related peptides. Antibodies raised to the N-terminal part of AKH (serum 433) and the central and C-terminal part (serum 241) were found to have different staining properties.

In the CC of the locust both antisera show a strong immunoreactivity with glandular cells, we therefore suggest that at least one of the compounds revealed is AKH. Some of the glandular cells in the locust and large numbers of glandular cells in the CC of the cockroach are revealed by the N-terminal specific antiserum. On the other hand, neurons in the central nervous system are revealed only by the C-terminal specific antiserum. The possible identity of the various substances revealed by these two antisera is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baumann E, Gersch M (1982) Purification and identification of neurohormone D, a heart accelerating peptide from the corpora cardiaca of the cockroach Periplaneta americana. Insect Biochem 12:7–14

    Google Scholar 

  • Carlsen J, Herman WS, Christensen M, Josefsson L (1979) Characterization of a second peptide with adipokinetic and red pigment-concentrating activity from the locust corpora cardiaca. Insect Biochem 9:497–501

    Google Scholar 

  • Eckert M (1977) Immunologische Untersuchungen des neuroendokrinen Systems von Insekten. IV. Differenzierte immunhistochemische Darstellung von Neurosekreten des Gehirns und der Corpora cardiaca bei der Schabe Periplaneta americana. Zool Jb Physiol 81:25–41

    Google Scholar 

  • Gäde G (1984) Adipokinetic and hyperglycaemic factors of different insect species: separation with high performance liquid chromatography. J Insect Physiol 30:729–736

    Google Scholar 

  • Goldsworthy GJ, Gäde G (1983) The chemistry of hypertrehalosemic factors. In: Downer RGH, Laufer H (eds) Endocrinology of insects. Vol 1. AR Liss, Inc, New York, pp 109–119

    Google Scholar 

  • Greenberg MJ, Price DA, Lehman HK (1985) FMRFamide-like peptides of molluscs and vertebrates: distribution and evidences of function. In: Kobayashi H et al. (eds) Neurosecretion and the biology of neuropeptides. Japan Sci Soc Press, Tokyo/Springer-Verlag, Berlin, pp 370–376

    Google Scholar 

  • Holwerda DA, Weeda E, van Doorn JM (1977) Separation of the hyperglycemic and adipokinetic factors from the cockroach corpus cardiacum. Insect Biochem 7:477–481

    Google Scholar 

  • Krieger DT, Liotta AS (1979) Pituitary hormones in brain: where, how and why? Science (Wash) 205:366–372

    Google Scholar 

  • Mordue W (1981) Adipokinetic hormone and related peptides. In: Farner DS, Lederis K (eds) Neurosecretion. Molecules, cells, systems. Springer, Berlin, pp 391–401

    Google Scholar 

  • Mordue W, Morgan PJ (1984) Isolation and characterization of neurohormones from locusts. In: Borkoveč AB, Kelly TJ (eds) Insect neurochemistry and neurophysiology. Plenum Publ Corp, New York & London, pp 77–91

    Google Scholar 

  • Nakane PK (1968) Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method. A study on pituitary gland of the rat. J Histochem Cytochem 16:557–558

    Google Scholar 

  • Nakanishi S, Inoue A, Kita T, Nakamura M, Chang ACY, Cohen SN, Numa S (1979) Nucleotide sequence of cloned cDNA for bovine corticotropin-β-lipotropin precursor. Nature (Lond) 278:423–427

    Google Scholar 

  • O'Shea M, Witten J, Schaffer M (1984) Isolation and characterization of two myoactive neuropeptides: further evidence of an invertebrate peptide family. J Neurosci 4:521–529

    Google Scholar 

  • Raabe M (1982) Insect neurohormones. Plenum Press, New York, London, xiv + 352 pp

    Google Scholar 

  • Scarborough RM, Jamieson GC, Kalish F, Kramer SJ, McEnroe GA, Miller CA, Schooley DA (1984) Isolation and primary structure of two peptides with cardioaccelerating and hyperglycaemic activity from the corpora cardiaca of Periplaneta americana. Proc Natl Acad Sci (US): 5575–5579

  • Scharrer B (1977) Peptides in neurobiology: historical introduction. In: Gainer H (ed) Peptides in neurobiology, Plenum Press, New York, London, pp 1–8

    Google Scholar 

  • Scharrer B (1978) Peptidergic neurons: facts and trends. Gen Comp Endocrinol 34:50–62

    Google Scholar 

  • Scheller RH, McAllister JB (1983) Molecular cloning of a multigene family encoding neuropeptides which govern egg-laying in Aplysia. In: Lever J, Boer HH (eds) Molluscan neuroendocrinology. North-Holland Publ. Co. Amsterdam, pp 38–44

    Google Scholar 

  • Schooneveld H, Tesser GI, Veenstra JA, Romberg-Privee HM (1983) Adipokinetic hormone and AKH-like peptide demonstrated in the corpora cardiaca and nervous system of Locusta migratoria by immunocytochemistry. Cell Tissue Res 230:67–76

    Google Scholar 

  • Schooneveld H, Romberg-Privee HM, Veenstra JA (1985) Adipokinetic hormone-immunoreactive peptide in the endocrine and central nervous system of several insect species: a comparative immunocytochemical approach. Gen Comp Endocrinol 57:184–194

    Google Scholar 

  • Sternberger LA (1979) Immunocytochemistry. John Wiley & Sons, New York, pp 354

    Google Scholar 

  • Stone JV, Mordue W, Batley KE, Morris HR (1976) Structure of locust adipokinetic hormone, a neurohormone that regulates lipid utilisation during flight. Nature (London) 263:207–211

    Google Scholar 

  • Swaab DF, Pool CW, van Leeuwen FW (1977) Can specificity ever be proved in immunocytochemical staining? J Histochem Cytochem 25:388–391

    Google Scholar 

  • Veenstra JA, Romberg-Privee HM, Schooneveld H, Polak JM (1985) Immunocytochemical localization of peptidergic neurons and neurosecretory cells in the neuroendocrine system of the Colorado potato beetle with antisera to vertebrate regulatory peptides. Histochemistry 82:9–18

    Google Scholar 

  • Witten J, Schaffer MH, O'Shea M (1983) Structure and biology of two new related neuropeptides from insect: Further evidence for a peptide family. Soc Neurosci Abstr 9:313

    Google Scholar 

  • Witten J, Schaffer MH, O'Shea M, Carter Cook J, Hemling ME, Rinehart KL Jr (1984) Structures of two cockroach neuropeptides assigned by fast atom bombardment mass spectrometry. Biochem Biophys Res Comm 124:350–358

    Google Scholar 

  • Yamashiro D, Applebaum SW, Li CH (1984) Synthesis of shrimp red pigment concentrating hormone analogs and their biological activity in locusts. Int J Pept Protein Res 23:39–41

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schooneveld, H., Romberg-Privee, H.M. & Veenstra, J.A. Immunocytochemical differentiation between adipokinetic hormone (AKH)-like peptides in neurons and glandular cells in the corpus cardiacum of Locusta migratoria and Periplaneta americana with C-terminal and N-terminal specific antisera to AKH. Cell Tissue Res. 243, 9–14 (1986). https://doi.org/10.1007/BF00221847

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00221847

Key words

Navigation