Summary
Apple (Malus domestica) transgenic T1 was obtained byAgrobacterium tumefaciens-mediated transformation of Malling 26 rootstock using the plasmid binary vector pLDB 15. pLDB 15 contains within its T -DNA a gene encoding the lytic protein attacin E. The integration of the attacin E gene into the apple genome was confirmed by Southern analysis. Northern analysis indicated the presence of an attacin E mRNA in plants inoculated withErwinia amylovora. After inoculation ofin vitro grown plants of T1, Malling 26, and Malling 7 (resistant control) withE. amylovora, the loglo of the inoculum concentration lethal to 50% of the plants was 5.4, 4.4, and 5.6, respectively. In greenhouse trials for resistance to fire blight, T1 was significantly more resistant than ‘Mailing 26’.
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Abbreviations
- DNA:
-
deoxyribonucleicacid
- GUS:
-
beta-glucuronidase
- M.7:
-
Malling7
- M.26:
-
Malling26
- MU:
-
4-methylumbelliferone
- MUG:
-
4-methylumbelliferyl-beta-D-glucuronide
- nptII:
-
neomycin phosphotransferase gene
- RNA:
-
ribonucleic acid
- X-glu:
-
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronic acid
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Norelli, J.L., Aldwinckle, H.S., Destéfano-Beltrán, L. et al. Transgenic ‘Mailing 26’ apple expressing the attacin E gene has increased resistance toErwinia amylovora . Euphytica 77, 123–128 (1994). https://doi.org/10.1007/BF02551474
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DOI: https://doi.org/10.1007/BF02551474