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Hydrodynamic Cavitation–Assisted Oxidative Pretreatment and Sequential Production of Ethanol and Xylitol as Innovative Approaches for Sugarcane Bagasse Biorefineries

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Abstract

In the present work, a new alternative of hydrodynamic cavitation–assisted pretreatment associated with an advanced oxidative process was proposed, together with a new approach to obtain bioproducts in sequential fermentations. Enzymatic hydrolysate of sugarcane bagasse (SCB) was fermented to ethanol by Saccharomyces cerevisiae. After distillation of this alcohol, the obtained vinasse was used to produce xylitol by Candida tropicalis. Influent variables in pretreatment were evaluated by performing experiments according to a statistical design, and, at optimum conditions (ozone flowrate of 10 mg/min, and H2O2 concentration of 0.61%), about 84% and 78% of glucan and xylan hydrolysis yields were obtained in enzymatic hydrolysis, respectively. In the sequential fermentations for ethanol and xylitol production, yield values of 0.41 g/g and 0.55 g/g, respectively, were obtained, with corresponding volumetric productivities of 8.33 g/Lh and 0.64 g/Lh, respectively. The proposed strategy was shown as a promising approach for biorefineries, considering the mild conditions of pretreatment and the possibility of high ethanol production using S. cerevisiae in a fermentation process similar to that one already available in sucro-alcoholic sector, followed by xylitol production in vinasse-based medium.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors also gratefully acknowledge the Fundação de Amparo à Pesquisa do Estado de São Paulo (grant #2016/10636-8, grant #2020/12059-3, grant#2020/16638-8 São Paulo Research Foundation – FAPESP) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil), for financial support. Besides, the authors also thank Ipiranga Agroindustrial-LTDA. (Descalvado, São Paulo, Brazil), by the donation of sugarcane bagasse sample, and the UFMG for the yeast donation.

Funding

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior –Brasil (CAPES) Finance code 001, and also by Fundação de Amparo à Pesquisa do Estado de São Paulo (grant #2016/10636-8, grant #2020/12059-3, grant#2020/16638-8, São Paulo Research Foundation – FAPESP), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil).

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Prado, C.A., Cunha, M.L.S., Terán-Hilares, R. et al. Hydrodynamic Cavitation–Assisted Oxidative Pretreatment and Sequential Production of Ethanol and Xylitol as Innovative Approaches for Sugarcane Bagasse Biorefineries. Bioenerg. Res. 16, 2229–2241 (2023). https://doi.org/10.1007/s12155-022-10555-6

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