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Localization of dopamine D2 receptor mRNA in glomus cells of the rabbit carotid body byin situ hybridization

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Journal of Neurocytology

Summary

The localization of mRNA coding for the dopamine D2 receptor was studied in the rabbit carotid body usingin situ hybridization with synthetic35S-labelled oligodeoxynucleotides. Using autoradiography on cryostat or semi-thin sections, labelling was observed over the cytoplasm of glomus cells, but not over sustentacular cells. A quantitative study showed that labelling intensity (silver grain density) was increased by haloperidol treatment. These results suggest that glomus cells express the dopamine D2 receptor gene and that this expression is regulated.

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Authors and Affiliations

  1. Laboratoire de Cytologie, Université de Bordeaux II et USA CNRS 339, Avenue des Facultes, 33405, Talence Cedex, France

    A. Verna & A. Schamel

  2. Laboratoire d'Histologie-Embryologie, Université de Bordeaux II et URA CNRS 1200, 146 rue Léo Saignat, 33076, Bordeaux Cedex, France

    C. Le Moine & B. Bloch

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  1. A. Verna
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  2. A. Schamel
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  3. C. Le Moine
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  4. B. Bloch
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Verna, A., Schamel, A., Le Moine, C. et al. Localization of dopamine D2 receptor mRNA in glomus cells of the rabbit carotid body byin situ hybridization. J Neurocytol 24, 265–270 (1995). https://doi.org/10.1007/BF01186539

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  • DOI: https://doi.org/10.1007/BF01186539

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