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Functional multineuron calcium imaging for systems pharmacology

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Abstract

Functional multineuron calcium imaging (fMCI) is a large-scale technique used to access brain function on a single-neuron scale. It detects the activity of individual neurons by imaging action potential-evoked transient calcium influxes into their cell bodies. fMCI has recently been used as a high-throughput research tool to examine how neuronal activity is altered in animal models of brain diseases, for example stroke, Alzheimer’s disease, and epilepsy, and to estimate how pharmacological agents act on normal and abnormal states of neuronal networks. It offers unique opportunities to discover the mechanisms underlying neurological disorders and new therapeutic targets.

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Author information

Authors and Affiliations

  1. Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan

    Naoya Takahashi, Yuji Takahara, Daisuke Ishikawa, Norio Matsuki & Yuji Ikegaya

  2. Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, 5 Sanbancho Chiyoda-ku, Tokyo, 102-0075, Japan

    Yuji Ikegaya

Authors
  1. Naoya Takahashi
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  2. Yuji Takahara
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  3. Daisuke Ishikawa
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  4. Norio Matsuki
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  5. Yuji Ikegaya
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Corresponding author

Correspondence to Yuji Ikegaya.

Appendices

Appendix

Publication list of methods and tools for details

Loading procedure for calcium indicators

In vitro fMCI

(Bath application) Ikegaya et al, 2005 [54]; Takahashi et al., 2007 [55]

(Pressure injection) Stosiek et al., 2003 [21]

In vivo fMCI

(Pressure injection) Stosiek et al., 2003 [21]; Nimmejahn et al., 2004 [8]; Ohki et al, 2005 [22]

(Electroporation) Nevian and Helmchem, 2007 [56]; Nagayama et al., 2007 [24]

fMCI data analysis

Cell identification

(Fluorescence contour) Crepel et al., 2007 [57]

(Correlation based) Ozden et al. 2008 [58]

(Spatio-temporal independent component analysis) Mukamel et al., 2009 [59]

Extraction of calcium signals (spike detection)

(Temporal deconvolution) Yaksi and Friedrich, 2006 [33]

(Template matching) Kerr et al., 2005 [30]; Greenberg et al., 2008 [32]

(Machine learning) Sasaki et al., 2008 [7]

(Spatio-temporal independent component analysis) Mukamel et al., 2009 [59]

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Takahashi, N., Takahara, Y., Ishikawa, D. et al. Functional multineuron calcium imaging for systems pharmacology. Anal Bioanal Chem 398, 211–218 (2010). https://doi.org/10.1007/s00216-010-3740-6

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  • DOI: https://doi.org/10.1007/s00216-010-3740-6

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