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HPLC-DAD-ESI/MS identification of anthocyanins in Dioscorea trifida L. yam tubers (purple sachapapa)

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Abstract

The coloured tubers of purple sachapapa (Dioscorea trifida L.), a common crop in the subtropical and tropical regions, were analysed to characterise their anthocyanin composition. High-performance liquid chromatography-diode array detection-mass spectrometry (HPLC-DAD-ESI/MS) allowed the detection of 12 anthocyanin pigments. The compounds identified were derived from peonidin (Pn), cyanidin (Cy), and pelargonidin (Pg) aglycones, most of them showing the same substitution pattern, anthocyanin 3-acylglucoside-5-glucoside, where the acyl residue consisted of a hydroxycinnamic acid (i.e., ferulic or p-coumaric acid). The main anthocyanin found was peonidin 3-O-p-coumaroylglucoside-5-O-glucoside.

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Acknowledgments

The Grupo de Investigación en Polifenoles, Universidad de Salamanca (GIP-USAL) is financially supported by Junta de Castilla y León (GR133, Programa de Ayudas a los Grupos de Excelencia) and the Spanish Ministerio de Ciencia e Innovación (FUN-C-FOOD CSD2007-00063, Consolider-Ingenio 2010 Programme).

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Authors and Affiliations

  1. Centro de Investigación de Bioquímica y Nutrición, Facultad de Medicina Humana, Universidad de San Martín de Porres, Lima, 12, Peru

    Fernando Ramos-Escudero

  2. Grupo de Investigación en Polifenoles, Unidad de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno, 37007, Salamanca, Spain

    Celestino Santos-Buelga, José Joaquín Pérez-Alonso & Montserrat Dueñas

  3. Department of Pharmaceutical Sciences and Pharmacology and Toxicology, Washington State University, Pullman, Washington, 99164-6534, USA

    Jaime A. Yáñez

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  1. Fernando Ramos-Escudero
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  2. Celestino Santos-Buelga
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  3. José Joaquín Pérez-Alonso
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  4. Jaime A. Yáñez
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  5. Montserrat Dueñas
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Correspondence to Montserrat Dueñas.

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Ramos-Escudero, F., Santos-Buelga, C., Pérez-Alonso, J.J. et al. HPLC-DAD-ESI/MS identification of anthocyanins in Dioscorea trifida L. yam tubers (purple sachapapa). Eur Food Res Technol 230, 745–752 (2010). https://doi.org/10.1007/s00217-010-1219-5

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  • DOI: https://doi.org/10.1007/s00217-010-1219-5

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