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Evidence for cholesterol-lowering activity by Bifidobacterium bifidum PRL2010 through gut microbiota modulation

  • Applied microbial and cell physiology
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Abstract

Bifidobacteria are members of the human gut microbiota, which are known to influence the metabolic abilities of their host. Here, we investigated the capabilities of bifidobacteria to reduce cholesterol levels in synthetic growth media, clearly demonstrating assimilation of this molecule by particular bifidobacterial strains, including Bifidobacterium bifidum PRL2010 (LMG S-28692). The transcriptomic analysis of PRL2010 cells cultivated in the presence of cholesterol revealed a significantly increased transcription level of genes encoding putative transporters and reductases, indicative of specific mechanisms for cholesterol assimilation as well as cholesterol conversion to coprostanol. Cholesterol lowering activity of B. bifidum PRL2010 cells was further evaluated by means of an in vivo murine model, showing that the fecal microbiota of mice is modified toward those bacteria involved in the metabolism of cholesterol.

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Acknowledgments

We thank GenProbio srl for financial support of the Laboratory of Probiogenomics. This work was financially supported by a FEMS Jensen Award to FT. FT is a members of The Alimentary Pharmabiotic Centre, while DvS is also a member of the Alimentary Glycoscience Research Cluster, both funded by Science Foundation Ireland (SFI), through the Irish Government’s National Development Plan (Grant numbers SFI/12/RC/2273 and 08/SRC/B1393, respectively).

Conflict of interest

The authors declare no financial or commercial conflict of interest.

Compliance with the ethical standards

The authors declare that all the procedures used for murine experiments (housing and experiments) were conducted in accordance with the animal experimentation European Communities Council Directive of 24 November 1986 (86/EEC) and approved by the ethical commission of the University of Parma and by the Italian Institute of Health.

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  1. Borja Sanchez

    Present address: Department of Analytical Chemistry and Food Science, Faculty of Food Science and Technology, University of Vigo, Vigo, Spain

Authors and Affiliations

  1. Department of Pharmacy, University of Parma, Parma, Italy

    Ilaria Zanotti, Antonio Piemontese, Lisa Elviri & Bernini Franco

  2. Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland

    Francesca Turroni & Douwe van Sinderen

  3. Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy

    Leonardo Mancabelli, Christian Milani, Gilda Prevedini & Marco Ventura

  4. GenProbio srl, Parma, Italy

    Alice Viappiani

  5. Dairy Research Institute of Asturias, Spanish National Research Council (IPLA-CSIC), Villaviciosa, Asturias, Spain

    Borja Sanchez & Abelardo Margolles

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  1. Ilaria Zanotti
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Correspondence to Francesca Turroni.

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Ilaria Zanotti, Francesca Turroni, and Antonio Piemontese equally contributed to the work.

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Zanotti, I., Turroni, F., Piemontese, A. et al. Evidence for cholesterol-lowering activity by Bifidobacterium bifidum PRL2010 through gut microbiota modulation. Appl Microbiol Biotechnol 99, 6813–6829 (2015). https://doi.org/10.1007/s00253-015-6564-7

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