Abstract
To investigate the potential for estimating the time since death by monitoring the evolution of different metabolites in brain tissue by 1H-MRS, an animal model using pig heads was established. The maximum examination interval was 3 weeks. Within this time interval spectra revealed different compositions of metabolites, including metabolites observed in the normal brain and as products of bacterial decomposition processes (N-acetyl-aspartate 0–130 h, creatine 0–170 h, bound trimethylammonium, e. g. choline compounds, during the whole time course with fluctuating intensities, lactate 0–200 h, alanine and acetate during the whole time course, succinate and free trimethylammonium after approx. 100 h postmortem). The proposed approach may offer a new method to estimate later postmortem intervals although these observations have to be confirmed by further studies.
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References
Amendt J, Krettek R, Niess C, Zehner R, Bratzke H (2000) Forensic entomology in Germany. Forensic Sci Int 113:309–314
Bourel B, Tournel G, Hedouin V, Gosset D (2004) Entomofauna of buried bodies in northern France. Int J Legal Med 118:215–220
Brinkmann B (2004) Entomology. Int J Legal Med 118:187
Cecil KM, Lenkinski RE, Meaney DF, McIntosh TK, Smith DH (1998) High-field proton magnetic resonance spectroscopy of a swine model for axonal injury. J Neurochem 70:2038–2044
Henssge C, Krompecher T (2002) The estimation of time since death in the early postmortem period, 2nd edition. Arnold, London
Ith M, Bigler P, Scheurer E, Kreis R, Hofmann L, Dirnhofer R, Boesch C (2002) Observation and identification of metabolites emerging during postmortem decomposition of brain tissue by means of in situ1H-magnetic resonance spectroscopy. Magn Reson Med 48:915–920
Kaneshrajah G, Turner B (2004) Calliphora vicina larvae grow at different rates on different body tissues. Int J Legal Med 118:242–244
Lai PH, Ho JT, Chen WL, Hsu SS, Wang JS, Pan HB, Yang CF (2002) Brain abscess and necrotic brain tumor: discrimination with proton MR spectroscopy and diffusion-weighted imaging. Am J Neuroradiol 23:1369–1377
Michaelis T, Helms G, Frahm J (1996) Metabolic alterations in brain autopsies: proton NMR identification of free glycerol. NMR Biomed 9:121–124
Zehner R, Amendt J, Schütt S, Sauer J, Krettek R, Povolny D (2004) Genetic identification of forensically important flesh flies (Diptera: Sarcophagidae). Int J Legal Med 118:245–247
Acknowledgement
The authors thank A. Gussew for excellent technical support during parts of the experiments.
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Banaschak, S., Rzanny, R., Reichenbach, J.R. et al. Estimation of postmortem metabolic changes in porcine brain tissue using 1H-MR spectroscopy—preliminary results. Int J Legal Med 119, 77–79 (2005). https://doi.org/10.1007/s00414-004-0495-7
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DOI: https://doi.org/10.1007/s00414-004-0495-7