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Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

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Abstract

Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.

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Acknowledgements

This study was partially supported by a NATO/Royal Society Postdoctoral Fellowship awarded to Dr. E. Spitalska. The experiments presented in this paper comply with the current UK laws.

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Authors and Affiliations

  1. School of Agriculture, Food and Rural Development, University of Newcastle, Newcastle upon Tyne , NE1 7RU, UK

    Eva Spitalska & Olivier A. E. Sparagano

  2. Slovak Academy of Sciences, Bratislava, Slovak Republic

    Eva Spitalska

  3. Istituto Zooprofilattico Sperimentale della Sicilia, 90124 , Palermo, Italy

    Alessandra Torina, Vincenza Cannella & Santo Caracappa

Authors
  1. Eva Spitalska
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  2. Alessandra Torina
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  3. Vincenza Cannella
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  4. Santo Caracappa
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  5. Olivier A. E. Sparagano
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Corresponding author

Correspondence to Olivier A. E. Sparagano.

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Spitalska, E., Torina, A., Cannella, V. et al. Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene. Parasitol Res 94, 318–320 (2004). https://doi.org/10.1007/s00436-004-1217-2

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  • DOI: https://doi.org/10.1007/s00436-004-1217-2

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