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Characterization of the carbohydrate components of Taenia solium oncosphere proteins and their role in the antigenicity

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Abstract

This study examines the carbohydrate composition of Taenia solium whole oncosphere antigens (WOAs), in order to improve the understanding of the antigenicity of the T. solium. Better knowledge of oncosphere antigens is crucial to accurately diagnose previous exposure to T. solium eggs and thus predict the development of neurocysticercosis. A set of seven lectins conjugates with wide carbohydrate specificity were used on parasite fixations and somatic extracts. Lectin fluorescence revealed that d-mannose, d-glucose, d-galactose and N-acetyl-d-galactosamine residues were the most abundant constituents of carbohydrate chains on the surface of T. solium oncosphere. Lectin blotting showed that posttranslational modification with N-glycosylation was abundant while little evidence of O-linked carbohydrates was observed. Chemical oxidation and enzymatic deglycosylation in situ were performed to investigate the immunoreactivity of the carbohydrate moieties. Linearizing or removing the carbohydrate moieties from the protein backbones did not diminish the immunoreactivity of these antigens, suggesting that a substantial part of the host immune response against T. solium oncosphere is directed against the peptide epitopes on the parasite antigens. Finally, using carbohydrate probes, we demonstrated for the first time that the presence of several lectins on the surface of the oncosphere was specific to carbohydrates found in intestinal mucus, suggesting a possible role in initial attachment of the parasite to host cells.

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Acknowledgments

Funding for this project came from the Gates project 23981, International Foundation for Science (IFS) grant B/4103-1 and the RG-ER anonymous fund for Tropical Research. We thank Dr. Eric Cossio, Dr. Carlos Carmona, Cecilia Casaravilla, and Dr. Holger Mayta and the administrative help of Ms. Marjory Meza and the technical assistance of MSc. Milagros Zavaleta.

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Authors and Affiliations

  1. Department of Cellular and Molecular Sciences, Faculty of Sciences and Philosophy, Universidad Peruana Cayetano Heredia, PO Box 5045, Lima, Peru

    Yanina Arana, Manuela Verastegui, Louis Grandjean, Hector H. Garcia & Robert H. Gilman

  2. Vaccine Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892-5065, USA

    Iskra Tuero

  3. Wellcome Centre for Clinical Tropical Medicine, Imperial College, St. Mary’s Campus, Praed Street, London, W2 1PG, UK

    Louis Grandjean

  4. Cysticercosis Unit, Instituto de Ciencias Neurologicas, Jr. Ancash 1271, Barrios Altos, Lima, Peru

    Hector H. Garcia

  5. Department of International Health, Johns Hopkins University, Bloomberg School of Hygiene and Public Health, Baltimore, 615 N. Wolfe Street Room W#5515, Baltimore, MD, 21205, USA

    Robert H. Gilman

  6. AB PRISMA, Calle Carlos Gonzales 251, San Miguel, Lima, 32, Peru

    Robert H. Gilman

Authors
  1. Yanina Arana
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  2. Manuela Verastegui
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  3. Iskra Tuero
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  4. Louis Grandjean
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  5. Hector H. Garcia
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  6. Robert H. Gilman
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Correspondence to Manuela Verastegui.

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Arana, Y., Verastegui, M., Tuero, I. et al. Characterization of the carbohydrate components of Taenia solium oncosphere proteins and their role in the antigenicity. Parasitol Res 112, 3569–3578 (2013). https://doi.org/10.1007/s00436-013-3542-9

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  • DOI: https://doi.org/10.1007/s00436-013-3542-9

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