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Biogas production: evaluation of the influence of K2FeO4 pretreatment of maple leaves (Acer platanoides) on microbial consortia composition

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Abstract

The potential of K2FeO4 as a pretreatment agent of a lignocellulosic material was examined on leaves of Acer platanodides as the sole substrate for biogas production by anaerobic digestion carried out through modelling laboratory-scaled semi-continuous reactors differing in loading rates and substrate (pretreated and untreated leaves). The quality of bioagas produced by K2FeO4-pretreated leaves was significantly better in terms of higher methane content and lower content of H2S. K2FeO4 had no crucial influence on growth inhibition of biogas-producing bacteria, which were analysed by comprehensive culture-independent methods utilising high-throughput sequencing of specific genes [bacterial and archaeal 16S rRNA, formyltetrahydrofolate synthetase gene (fhs), methyl-coenzyme M reductase α subunit gene (mcrA) and fungal internal transcribed spacers (ITS)]. The higher amount of CH4 in biogas utilising pretreated leaves as substrate could be caused by a shift to acetoclastic methanogenesis pathway, which was indicated by the higher amount of homoacetogenic bacteria and acetotrophic methanogens detected in those reactors.

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Acknowledgements

This study was mainly financed by SAS-MOST Joint Research Cooperation (Bilateral project Slovakia–Taiwan) under Grant nos. MOST 104-2923-E-006-001-MY3 and SAS-MOST JRP 2014/3. This work was also supported by other projects of the Slovak Research Agencies under the contracts no. APVV-16-0124, APVV-16-0171, VEGA 1/0543/15 and VEGA 1/0558/17.

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Authors and Affiliations

  1. Institute of Molecular Biology, Slovak Academy of Science, Dúbravská cesta 21, 84551, Bratislava, Slovakia

    Matej Planý, Lucia Kraková, Andrea Puškárová, Mária Bučková & Domenico Pangallo

  2. Department of Inorganic Technology, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 81237, Bratislava, Slovakia

    Marianna Czolderová

  3. Comenius University in Bratislava, Comenius University Science Park, Ilkovicova 8, 84104, Bratislava, Slovakia

    Katarína Šoltys & Tomáš Szemes

  4. Geneton s.r.o., Galvaniho 7, 82104, Bratislava, Slovakia

    Jaroslav Budiš & Tomáš Szemes

  5. Department of Computer Science, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava, Mlynska dolina, 84248, Bratislava, Slovakia

    Jaroslav Budiš

  6. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina Ilkovičova 6, 84215, Bratislava, Slovakia

    Tomáš Szemes

  7. Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 81237, Bratislava, Slovakia

    Tomáš Mackulak

  8. Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, East District, Tainan City, 701, Taiwan, Republic of China

    Jer-Horng Wu

  9. Caravella s.r.o., Tupolevova 2, 85101, Bratislava, Slovakia

    Domenico Pangallo

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Planý, M., Czolderová, M., Kraková, L. et al. Biogas production: evaluation of the influence of K2FeO4 pretreatment of maple leaves (Acer platanoides) on microbial consortia composition. Bioprocess Biosyst Eng 42, 1151–1163 (2019). https://doi.org/10.1007/s00449-019-02112-x

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