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Bovine subclinical ketosis in dairy herds in Nishaboor, Iran

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Abstract

Ketosis causes economic losses to the dairy industry. Subclinical ketosis (SCK) is defined as elevated concentrations of circulating ketone bodies in the absence of clinical signs. Measurement of blood β-hydroxy butyrate (BHB) concentration is considered as the gold standard method for herd monitoring. The objective of this study was to determine the prevalence of SCK in postpartum cows in Nishaboor, Iran. A total of 109 lactating cows in 2–4 weeks of lactation from 15 herds in Nishaboor were included in this study. Serum BHB and glucose concentrations were determined by BHB dehydrogenase and glucose oxidase methods. At the cutoff point of 1,400 μmol/L of BHB concentration, 18.42% (35/190) of dairy cows were considered subclinical ketotic with the herd range of 0–50%. The mean concentrations of glucose and BHB in ketotic were 30.6 ± 1.44 mg/dL and 2,400 ± 420 μmol/L, and in nonketotic cows were 43.6 ± 0.7 mg/dL and 637 ± 80 μmol/L, respectively. Blood glucose concentration in subclinical ketotic cows were significantly lower (p ≤ 0.05) than in nonketotic cows. The serum concentrations of BHB and glucose in the tested cows were significantly (p ≤ 0.01) and inversely correlated (r = 0.457, p ≤ 0.05). The results of this study showed that the prevalence of SCK in Iran is higher rather than those reported from other countries. In order to reduce economic losses of SCK disease performing regular monitoring metabolic tests during transition period is highly recommended.

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References

  • Andersson L (1984) Detection, occurrence, causes and effects of hyperketonaemia in Swedish dairy cows. Doctoral thesis. SLU, Experimental Station, Veterinary Institute, Skara

  • Andersson L (1988) Subclinical ketosis in dairy cows. Vet Clin North Am Food Anim Pract 4:233–251

    PubMed  CAS  Google Scholar 

  • Baird GD (1982) Primary ketosis in the high-producing dairy cow: clinical and subclinical disorders, treatment, prevention, and outlook. J Dairy Sci 65:1–10

    Article  PubMed  CAS  Google Scholar 

  • Bauman DE, Crie WW (1980) Partitioning of nutrients during pregnancy and lactation: a review of mechanisms involving homeostasis and homeoresis. J Dairy Sci 63:1514–1529

    Article  PubMed  CAS  Google Scholar 

  • Bell AW (1995) Regulation of organic nutrient metabolism during transition from late pregnancy to early lactation. J Anim Sci 73:2804–2819

    PubMed  CAS  Google Scholar 

  • Detilleux JC, Gröhn YT, Quaas RL (1994) Effects of clinical ketosis on test day milk yields in Finnish Ayrshire cattle. J Dairy Sci 77:3316–3323

    Article  PubMed  CAS  Google Scholar 

  • Dohoo IR, Martin SW (1984a) Subclinical ketosis: prevalence and association with production and disease. Can J Comp Med 48:1–5

    PubMed  CAS  Google Scholar 

  • Dohoo IR, Martin SW (1984b) Disease, production and culling in Holstein Friesian cows. III. Disease and production as determinants of disease. Prev Vet Med 2:671–690

    Article  Google Scholar 

  • Drackley JK (1999) ADSA Foundation Scholar Award. Biology of dairy cows during the transition period: the final frontier? Review. J Dairy Sci 82:2259–2273

    Article  PubMed  CAS  Google Scholar 

  • Duffield TF (1997) Effects of a monensin controlled release capsule on energy metabolism, health, and production in lactating dairy cattle. Thesis dissertation. Guelph (Ontario): University of Guelph

  • Duffield TF (2000) Subclinical ketosis in lactating dairy cattle: metabolic disorders of ruminants. Vet Clin North Am Food Anim Pract 16:231–253

    PubMed  CAS  Google Scholar 

  • Duffield TF (2004) Monitoring strategies for metabolic disease in transition dairy cows. Proceedings of the WBC Congress, Québec

    Google Scholar 

  • Duffield TF, Sandals D, Leslie KE, Lissemore K, McBride BW, Lumsden JH, Dick P, Bagg R (1998) Efficacy of monensin for the prevention of subclinical ketosis in lactating dairy cows. J Dairy Sci 81:2866–7283

    Article  PubMed  CAS  Google Scholar 

  • Enjalbert F, Nicot MC, Bayourthe C, Moncoulon R (2001) Ketone bodies in milk and blood of dairy cows: relationship between concentrations and utilization for detection of subclinical ketosis. J Dairy Sci 84:583–589

    Article  PubMed  CAS  Google Scholar 

  • Geishauser T, Leslie K, Kelton D, Duffield TF (1998) Evaluation of five cowside tests for use with milk to detect subclinical ketosis in dairy cows. J Dairy Sci 81:438–443

    Article  PubMed  CAS  Google Scholar 

  • Geishauser T, Leslie K, Tenhag K, Bashiri A (2000) Evaluation of eight cow-side ketone tests in milk for detection of subclinical ketosis in dairy cows. J Dairy Sci 83:296–299

    Article  PubMed  CAS  Google Scholar 

  • Geishauser T, Leslie K, Kelton D, Duffield T (2001) Monitoring for subclinical ketosis in dairy herds. Compend Contin Educ Pract Vet 23:S65–S70

    Google Scholar 

  • Grohn Y, Lindberg LA, Bruss ML, Farvar TB (1983) Fatty infiltration of liver in spontaneously ketotic dairy cows. J Dairy Sci 66:2320–2328

    Article  PubMed  CAS  Google Scholar 

  • Jorritsma R, Baldee SJC, Schukken YH, Wensing T, Wentink GH (1998) Evaluation of a milk test for detection of subclinical ketosis. Vet Q 20:108–110

    Article  PubMed  CAS  Google Scholar 

  • Miettinen PVA (1990) Metabolic balance and reproductive performance in Finnish dairy cows. J Veterinary Med Ser A 37:417–424

    Article  CAS  Google Scholar 

  • Miettinen PVA (1994) Relationship between milk acetone and milk yield in individual cows. J Veterinary Med Ser A 41:102–109

    Article  CAS  Google Scholar 

  • Miettinen PV, Rainio VA, Kukkonen SA, Setälä JJ (1991) Finnish dairy cows: energy balance and seasonal variation in fertility. J Veterinary Med Ser A 38:427–4320

    Article  CAS  Google Scholar 

  • Oetzel GR (2003) Herd-based biological testing for metabolic disorders. Preconvention seminar. Am Assoc Bov Pract 36:1–16

    Google Scholar 

  • Oetzel GR (2004) Monitoring and testing dairy herds for metabolic disease. Vet Clin North Am Food Anim Pract 20:651–674

    Article  PubMed  Google Scholar 

  • Overton R, Bernal-Santos G, Perfield JD II, Bauman DE (2001) Effects of feeding conjugated linoleic fatty acids (CLA) on metabolism and performance of transition dairy cows. Cornell Nutr Conf 63:179–187

    Google Scholar 

  • Plym Forshell K, Andersson L, Pehrson P (1991) The relationship between the fertility of dairy cows and clinical and bio-chemical measurements, with special reference to plasma glucose and milk acetone. J Veterinary Med Ser A 38:608–616

    Article  CAS  Google Scholar 

  • Radostis OM, Gay CC (2008) Metabolic diseases. In: Radostitis OM, Gay CC, Hinchcliff KW, Contable P (eds) Veterinary medicine. A textbook of the diseases of cattle, horses, sheep, pigs and goats, 10th edn. Elsevier, London, p 1613

    Google Scholar 

  • Sakha M, Ameri M, Rohbakhsh A (2006) Changes in blood ß-hydroxybutyrate and glucose concentrations during dry and lactation periods in Iranian Holstein cows. Comp Clin Pathol 15:221–226

    Article  CAS  Google Scholar 

  • Sakha M, Ameri M, Sharifi H, Taheri I (2007) Bovine subclinical ketosis in dairy herds in Iran. Vet Res Commun 31:673–679

    Article  PubMed  CAS  Google Scholar 

  • Whitaker DA, Kelly JM, Smith EJ (1983) Subclinical ketosis and serum beta-hydroxybutyrate levels in dairy cattle. Br Vet J 139:462–463

    PubMed  CAS  Google Scholar 

  • Whitaker DA, Smith EJ, da Rosa GO, Kelly JM (1993) Some effects of nutrition and management on the fertility of dairy cattle. Vet Rec 133:61–64

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgment

The authors would like to thank Dr. Mehrdad Ameri for help in preparation of this manuscript.

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Authors and Affiliations

  1. Faculty of Veterinary Medicine, Department of Clinical Science, Islamic Azad University—Garmsar Branch, Garmsar, Iran

    Maysam Tehrani-Sharif

  2. Islamic Azad University—Garmsar Branch, Garmsar, Iran

    Masoud Hadadi, Hojat Hejazi Noughabi, Abolfazl Mohammadi & Farid Rostami

  3. Faculty of Veterinary Medicine, Department of Food & Hygiene, Shahid Bahonar University, Kerman, Iran

    Hamid Sharifi

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  1. Maysam Tehrani-Sharif
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  2. Masoud Hadadi
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  3. Hojat Hejazi Noughabi
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  4. Abolfazl Mohammadi
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  5. Farid Rostami
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  6. Hamid Sharifi
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Correspondence to Maysam Tehrani-Sharif.

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Tehrani-Sharif, M., Hadadi, M., Noughabi, H.H. et al. Bovine subclinical ketosis in dairy herds in Nishaboor, Iran. Comp Clin Pathol 21, 1637–1641 (2012). https://doi.org/10.1007/s00580-011-1340-2

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  • DOI: https://doi.org/10.1007/s00580-011-1340-2

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