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Hysterothylacium amoyense in Platycephalous indicus: a Persian Gulf fish and its experimental infection of mouse model

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Abstract

Anisakids belong to a genus of parasitic nematodes with a life cycle involving fish and marine mammals. They cause anisakidosis (anisakiasis) by accidental ingestion of larval stage (Anisakis and other Anisakids), causing both gastrointestinal and allergic reaction in human. The aim of the current study was to report the finding of Hysterothylacium amoyense in Platycephalous indicus fishes and also its probable pathologic effect on a mouse model. In this regard, a total of 27 live third stage larvae ranging from 1.5 to 3 mm in length were collected from gut submucosa of the fish Platycephalous indicus [ZAMIN KAN] and washed up by saline 6 %, and two were characterized as Hysterothylacium amoyense by morphologic and molecular techniques. A total of 25 larvae were orally fed to 5 inbred mice (each 5). A total of 5 mice were also selected as control group; they did not receive the larvae. After 2 weeks, all mice were necropsied using ethical protocol of SUMS. Their specimens, both gross and histologic, were observed and described. Tumor-like nodules were observed in 3 out of 5 infected mice group which could be misdiagnosed as cancer. Microscopic examinations showed lymphocytic infiltration. No pathologic changes were found in the control group. The results showed an invasive anisakid, Hysterthylacium amoyense, with histopathologic features observed in the mammal model (mouse). The parasite has been found in benthic fish Platycephalous indicus in the Persian Gulf, Bushehr region, southern Iran. The parasite could be regarded as a possible source of anisakidosis in coastal populations and should be regarded as a public health problem.

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Acknowledgments

This project was financially supported by the office of vice-chancellor for research at Shiraz University of Medical Sciences, Grant No: 93-7252. Technical assistance of Mr. Abouzar Valizadeh and Mr. Mohammad Mehdi Ommati are acknowledged. The authors thank Prof. S. Sajjadi for his valuable assistance.

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Authors and Affiliations

  1. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

    Mohsen Najjari, Seyed Mahmoud Sadjjadi & Mohammad Ebrahimipour

  2. Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Seyed Mahmoud Sadjjadi & Amin Derakhshanfar

  3. Center of Comparative & Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

    Amin Derakhshanfar

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  1. Mohsen Najjari
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  2. Seyed Mahmoud Sadjjadi
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Correspondence to Seyed Mahmoud Sadjjadi.

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Research involving human participants and/or animals

This protocol was performed in accordance with the international guiding principles for biomedical researchers involving laboratory animals at Shiraz University of Medical Sciences, Shiraz, Iran (code: ec:9372-7252).

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The present study had no tests on human.

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Najjari, M., Sadjjadi, S.M., Derakhshanfar, A. et al. Hysterothylacium amoyense in Platycephalous indicus: a Persian Gulf fish and its experimental infection of mouse model. Comp Clin Pathol 25, 1143–1149 (2016). https://doi.org/10.1007/s00580-016-2318-x

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  • DOI: https://doi.org/10.1007/s00580-016-2318-x

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