Abstract
Eugenia uniflora is a plant native to tropical America that holds great ecological and economic importance. The complete chloroplast (cp) genome sequence of Eugenia uniflora, a member of the Neotropical Myrtaceae family, is reported here. The genome is 158,445 bp in length and exhibits a typical quadripartite structure of the large (LSC, 87,459 bp) and small (SSC, 18,318 bp) single-copy regions, separated by a pair of inverted repeats (IRs, 26,334 bp). It contains 111 unique genes, including 77 protein-coding genes, 30 tRNAs and 4 rRNAs. The genome structure, gene order, GC content and codon usage are similar to the typical angiosperm cp genomes. Comparison of the entire cp genomes of E. uniflora L. and three other Myrtaceae revealed an expansion of 43 bp in the intergenic spacer located between the IRA/large single-copy (LSC) border and the first gene of LSC region. Simple sequence repeat (SSR) analysis revealed that most SSRs are AT rich, which contribute to the overall AT richness of the cp genome. Additionally, fewer SSRs are distributed in the protein-coding sequences compared to the noncoding regions. Phylogenetic analysis among 58 species based on 57 cp genes demonstrated a closer relationship between E. uniflora L. and Syzygium cumini (L). Skeels compared to the Eucalyptus clade in the Myrtaceae family. The complete cp genome sequence of E. uniflora reported here has importance for population genetics, as well as phylogenetic and evolutionary studies in this species and other Myrtaceae species from Neotropical regions.
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Acknowledgements
We would like to thank Prof. Andreia Turchetto Zolet for providing very helpful suggestions to our manuscript and Steven Clipman for correcting the English.
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This study was carried out with the support of FAPERGS and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
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Online Resource 1 List of accession numbers of the chloroplast genome sequences used to filter reads from cp genomes.
Online Resource 2 List of the 57 genes used in the ML and Bayesian phylogenetic analysis.
Online Resource 3 List of plastome sequences of Rosids included in the ML and Bayesian phylogenetic analyses.
Online Resource 4 Phylogenetic tree among 58 eurosids based on 57 protein-coding genes reconstructed by the maximum likelihood method.
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Eguiluz, M., Rodrigues, N.F., Guzman, F. et al. The chloroplast genome sequence from Eugenia uniflora, a Myrtaceae from Neotropics. Plant Syst Evol 303, 1199–1212 (2017). https://doi.org/10.1007/s00606-017-1431-x
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DOI: https://doi.org/10.1007/s00606-017-1431-x