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Molecular differentiation and phylogenetic analysis of the Egyptian foot-and-mouth disease virus SAT2

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Abstract

In February 2012, a massive new foot-and-mouth disease (FMD) outbreak struck Egypt. In this work, one-step RT-PCR assays were used for in-house detection and differentiation of foot-and-mouth disease virus (FMDV) in Egypt in this year using pan-serotypic and serotype-targeting sequence primers. FMDV SAT2 was the dominant virus in the examined isolates from the epidemic. The complete VP1 coding regions of two isolates were sequenced. The two isolates had 99.2 % sequence identity to most contemporary Egyptian SAT2 reference viruses, whereas they had 89.7-90.1 % identity to the SAT2/EGY/2/2012 isolate, which was collected from Alexandria, Egypt, and previously sequenced by WRLFMD. Phylogenetic analysis showed that Egypt had one topotype and two lineage of FMDV SAT2 in 2012. The Egyptian and the Palestinian 2012 strains were associated mainly with topotype VII, lineage SAT2/VII/Ghb-12, while the virus isolated from Alexandria Governorate belonged to the SAT2/VII/Alx-12 lineage. Topotype VII also comprised lineages that included strains isolated from Libya in 2012 and 2003. Furthermore, within the same topotype, the Egyptian SAT2/2012 isolates were related to strains from Saudi Arabia, Sudan, Eritrea, Cameroon and Nigeria. Nevertheless, more epidemiological work with neighboring countries is needed to prevent cross-border spread of disease and to reach a precise conclusion about the origin of the 2012 FMDV SAT2 emergency in the Middle East.

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References

  1. James AD, Rushton J (2002) The economics of foot and mouth disease. Rev Sci Tech Off Int Epiz 21(3):637–644

    CAS  Google Scholar 

  2. Arzt J, Juleff N, Zhang Z, Rodriguez LL (2011) The pathogenesis of foot-and-mouth disease I: viral pathways in cattle. Transbound Emerg Dis 58:291–304

    Article  CAS  PubMed  Google Scholar 

  3. Vallée H, Carré H (1922) Sur la pluralite du virus aphteux. CR Hebd Acad Sci Paris 174:1498–1500

    Google Scholar 

  4. Waldmann O, Trautwein K (1926) Experimentalle Untersuchungen ueber die Pluralitel des Maul und Klauenseuche. Berl Iteraztl Wschr 42:569–571

    Google Scholar 

  5. Brooksby JB (1958) The virus of foot and mouth disease. Adv Virus Res 5:1–37

    Article  CAS  PubMed  Google Scholar 

  6. Bastos ADS, Haydon DT, Sangare O, Boshof CIf, Edrich JL, Thomson GR (2003) The implications of virus diversity within the SAT2 serotype for control of foot-and-mouth disease in sub-Saharan Africa. J Gen Virol 84:1595–1606

    Article  CAS  PubMed  Google Scholar 

  7. Ahmed HA, Salem SA, Habashi AR, Arafa AA, Aggour MG, Salem GH, Gaber AS, Selem O, Abdelkader SH, Knowles NJ, Madi M, Valdazo-González B, Wadsworth J, Hutchings GH, Mioulet V, Hammond JM, King DP (2012) Emergence of foot-and-mouth disease virus SAT2 in Egypt during 2012. Transbound Emerg Dis 59(6):476–481

    Article  CAS  PubMed  Google Scholar 

  8. Kandeil A, El-Shesheny R, Kayali G, Moatasim Y, Bagato O, Darwish M, Gaffar A, Younes A, Farag T, Kutkat MA, Ali MA (2013) Characterization of the recent outbreak of foot-and-mouth disease virus serotype SAT2 in Egypt. Arch Virol 158:619–627

    Article  CAS  PubMed  Google Scholar 

  9. Beck E, Feil G, Strohmaierl K (1983) The molecular basis of the antigenic variation of foot-and-mouth disease virus. EMBO J 2(4):555–559

    CAS  PubMed Central  PubMed  Google Scholar 

  10. Kitson JDA, McCahon D, Belsham GJ (1990) Sequence analysis of monoclonal antibody resistant mutants of type O foot-and-mouth disease virus: evidence for the involvement of the three surface exposed capsid protein in four antigenic sites. Virology 179:26–34

    Article  CAS  PubMed  Google Scholar 

  11. Nunez JI, Fusi P, Borrego B, Brocchi E, Pacciarini ML, Sobrino F (2006) Genomic and antigenic characterization of viruses from the 1993 Italian foot-and mouth disease outbreak. Arch Virol 151:127–142

    Article  CAS  PubMed  Google Scholar 

  12. Aidaros HA (2002) Regional status and approaches to control and eradication of foot and mouth disease in the Middle East and North Africa. Rev Sci Tech Off Int Epiz 21(3):451–458

    CAS  Google Scholar 

  13. EL-Shehawy L, Abu-Elnaga H, Talat A, El-Garf E, Zakria A, Azab A (2011) A nucleotide sequencing of foot-and-mouth disease virus Egyptian strains. J Am Sci 7(7):430–435

    Google Scholar 

  14. Reid SM, Hutchings GH, Ferris NP, De Clercq K (1999) Diagnosis of foot and-mouth disease virus by RT-PCR: evaluation of primers for serotypic characterization of viral RNA in clinical samples. J Virol Methods 83:113–123

    Article  CAS  PubMed  Google Scholar 

  15. Shaw AE, Reid SM, Ebert K, Hutchings GH, Ferris NP, King DP (2007) Implementation of a one-step real-time RT-PCR protocol for diagnosis of foot-and-mouth disease. J Virol Methods 143(1):81–85

    Article  CAS  PubMed  Google Scholar 

  16. Saiz M, De La Morena DB, Blanco E, Nunez JI, Fernandez R, Sanchez-Vizkaino JM (2003) Detection of foot-and-mouth disease virus from culture and clinical samples by reverse transcription-PCR coupled to restriction enzyme and sequence analysis. Vet Res 34:105–117

    Article  CAS  PubMed  Google Scholar 

  17. Le VP, Lee KN, Nguyen T, Kim SM, Cho IS, Khang DD, Hien NB, Van Quyen D, Park JH (2012) A rapid molecular strategy for early detection and characterization of Vietnamese foot-and-mouth disease virus serotypes O, A, and Asia 1. J Virol Methods 180:1–6

    Article  CAS  PubMed  Google Scholar 

  18. Abu-Elnaga HI (2011) Single tube RT-PCR for simultaneous differentiation of foot-and-mouth disease virus O and A isolated in 2009 in Egypt. Egypt J Virol 8:30–52

    Google Scholar 

  19. Shin JH, Sohn HJ, Choi KS, Kwon BJ, Choi CU, Kim JH, Hwang EK, Park JH, Kim JY, Choi SH, Kim OK (2003) Identification and isolation of foot-and-mouth disease virus from primary suspect cases in Korea in 2000. J Vet Med Sci 65:1–7

    Article  CAS  PubMed  Google Scholar 

  20. Vangrysperre W, De Clercq K (1996) Rapid and sensitive polymerase chain reaction based detection and typing of foot-and-mouth disease virus in clinical samples and cell culture isolates, combined with a simultaneous differentiation with other genomically and:or symptomatically related viruses. Arch Virol 141:331–344

    Article  CAS  PubMed  Google Scholar 

  21. Knowles NJ, Samuel AR (1994) Polymerase chain reaction amplification and cycle sequencing of the 1D (VP1) gene of foot-and-mouth disease viruses. Paper presented at the session of the Research group of the standing Technical committee of European commission for the control of FMD, Vienna, 19–22 Sept 1994

  22. Ayelet G, Mahapatra M, Gelaye E, Egziabher BG, Rufeal T, Sahle M, Ferris NP, Wadsworth J, Hutchings GH, Knowles NJ (2009) Genetic characterization of foot-and-mouth disease viruses, Ethiopia, 1981-2007. Emerg Infect Dis 15(9):1409–1417

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  23. Azab AM, Abu-Elnaga HI, Zakria A (2012) Detection of foot-and-mouth disease virus (FMDV) by rRT-PCR in Egypt. Arab J Biotechnol 15(1):61–70

    Google Scholar 

  24. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  25. Amass SF, Pacheco JM, Mason PW, Schneider JL, Alvarez RM, Clark LK, Ragland D (2003) Procedures for preventing the transmission of foot and mouth disease virus to pigs and sheep by personnel in contact with infected pigs. Vet Rec 153:137–140

    Article  CAS  PubMed  Google Scholar 

  26. Abu-Elnaga HI (2008) Molecular studies of foot-and-mouth disease virus. PhD thesis, Zagazig University, Egypt

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Acknowledgments

This study is a part of the project entitled ‘molecular characterization of FMDV Egyptian strains for improvement of local produced vaccine’, ID number 1064. Science and Technology Development Fund (STDF), www.STDF.org.egy.

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Authors and Affiliations

  1. Department of Foot and Mouth Disease, Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt

    Laila I. EL-Shehawy, Hany I. Abu-Elnaga, Sonia A. Rizk, Ahmed S. Abd El-Kreem, A. A. Mohamed & Hossam G. Fawzy

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  1. Laila I. EL-Shehawy
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  2. Hany I. Abu-Elnaga
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  3. Sonia A. Rizk
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  4. Ahmed S. Abd El-Kreem
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  5. A. A. Mohamed
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  6. Hossam G. Fawzy
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Correspondence to Hany I. Abu-Elnaga.

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EL-Shehawy, L.I., Abu-Elnaga, H.I., Rizk, S.A. et al. Molecular differentiation and phylogenetic analysis of the Egyptian foot-and-mouth disease virus SAT2. Arch Virol 159, 437–443 (2014). https://doi.org/10.1007/s00705-013-1825-1

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  • DOI: https://doi.org/10.1007/s00705-013-1825-1

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