Abstract
The degree of stratification in the reticulorumen contents is considered a major explanatory factor for other morphological and physiological differences between ruminant feeding types. However, information on reticulorumen (RR) contents is usually gathered from dead animals or indirect such as passage kinetics. We hypothesized that, although the contents of the gastrointestinal tract can usually not be evaluated by ultrasonography, the three typical layers of stratified RR contents (gas dome, fibre mat, fluid) can be demonstrated by this technique. In three domestic cows, the gas dome in the cows’ rumens could be demonstrated sonographically by reverberation lines running in parallel to the line demarcating the rumen mucosa, as is typical for the sonographic image of large, gas-filled spaces. More ventrally, the area behind the rumen mucosa showed typical pattern indicating gaseous inclusions, corresponding to the fibre mat inside the rumen. Further ventrally, in two of the animals, the area behind the demarcation line appeared dark without reverberation lines, as is typical for large fluid-filled spaces. When the technique was applied to a captive, habituated, browse-fed moose, no gas dome could be demonstrated, supporting the hypothesis that the reticulorumen contents of browsers are less stratified. The results of this study indicate that sonography represents a useful tool for the demonstration of RR contents stratification in live animals.
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References
Ahvenjärvi S, Skiba B, Huhtanen P (2001) Effect of heterogenous digesta chemical composition on the accuracy of measurements of fibre flow in dairy cows. J Anim Sci 79:1611–1620
Beaumont R, Deswysen AG (1991) Mélange et propulsion du contenu du réticulo-rumen. Reprod Nutr Dev 31:335–359
Behrend A, Lechner-Doll M, Streich WJ, Clauss M (2004) Seasonal faecal excretion, gut fill, liquid and particle marker retention in mouflon (Ovis ammon musimon), and a comparison with roe deer (Capreolus capreolus). Acta Theriol 49:503–515
Bogner JR (1992) Artefakte. In: Zoller WG, Gresser U, Zöllner N (eds) Einführung in die Ultraschalldiagnostik. Karger, Basel, pp 14–21
Braun U, Götz M, Marmier O (1993) Ultrasonographic findings in cows with traumatic reticuloperitonitis. Vet Rec 133:416–422
Braun U, Flückiger M, Götz M (1994) Comparison of ultrasonographic and radiographic findings in cows with traumatic reticuloperitonitis. Vet Rec 135:470–478
Braun U, Götz M (1994) Ultrasonography of the reticulum in cows. Am J Vet Res 55:325–332
Braun U, Marmier O (1995) Ultrasonographic examinationn of the small intestine of cows. Vet Rec 136:239–244
Braun U, Pusterla N, Schönmann N (1995a) Ultrasonographic findings in cows with left displacement of the abomasum. Vet Rec 141:331–335
Braun U, Marmier O, Pusterla N (1995b) Ultrasonographic examination of the small intestine of cows with ileus of the duodenum, jejunum, or ileum. Vet Rec 137:209–215
Braun U, Wild K, Guscetti F (1997) Ultrasonographic examination of the abomasum of 50 cows. Vet Rec 140:93–98
Braun U, Iselin U, Lischer C, Fluri E (1998) Ultrasonographic findings in five cows before and after treatment of reticular abscesses. Vet Rec 142:184–189
Braun U, Amrein E (2001) Ultrasonographic examination of the caecum and proximal and spiral loop of the colon of cattle. Vet Rec 149:45–48
Braun U, Schweizer G, Flückiger M (2002a) Radiographic and ultrasonographic findings in three cows with reticulo-omasal obstruction due to a foreign body. Vet Rec 150:580–581
Braun U, Amrein E, Koller U, Lischer C (2002b) Ultrasonographic findings in cows with dilatation, torsion, and retroflexion of the caecum. Vet Rec 150:75–79
Braun U (2003) Ultrasonography in gastrointestinal disease in cattle. Vet J 166:112–124
Braun U, Blessing S (2006) Ultrasonographic examination of the omasum in 30 healthy cows. Vet Rec 159:812–815
Clarke RT, Reid CS (1974) Foamy bloat of cattle. A review. J Dairy Sci 57:753–785
Clauss M, Lechner-Doll M (2001) Differences in selective reticulo-ruminal particle retention as a key factor in ruminant diversification. Oecologia 129:321–327
Clauss M, Lechner-Doll M, Behrend A, Lason K, Lang D, Streich WJ (2001) Particle retention in the forestomach of a browsing ruminant, the roe deer (Capreolus capreolus). Acta Theriol 46:103–107
Clauss M, Lechner-Doll M, Streich WJ (2003) Ruminant diversification as an adaptation to the physicomechanical characteristics of forage. A reevaluation of an old debate and a new hypothesis. Oikos 102:253–262
Clauss M, Hummel J, Streich WJ (2006a) The dissociation of the fluid and particle phase in the forestomach as a physiological characteristic of large grazing ruminants: an evaluation of available, comparable ruminant passage data. Eur J Wildlife Res 52:88–98
Clauss M, Hofmann RR, Hummel J, Adamczewski J, Nygren K, Pitra C, Reese S (2006b) The macroscopic anatomy of the omasum of free-ranging moose (Alces alces) and muskoxen (Ovibos moschatus) and a comparison of the omasal laminal surface area in 34 ruminant species. J Zool Lond 270:346–358
Clauss M, Kaiser T, Hummel J (2007) The morphophysiological adaptations of browsing and grazing mammals. In: Gordon IJ, Prins HHT (eds) The ecology of browsing and grazing. Springer, Heidelberg, pp 47–88
Grau H (1955) Zur Funktion der Vormägen, besonders des Netzmagens, der Wiederkäuer. Berl Münch Tierärztl Wochenschr 15:271–275
Hofmann RR (1969) Zur Topographie und Morphologie des Wiederkäuermagens im Hinblick auf seine Funktion (nach vergleichenden Untersuchungen an Material ostafrikanischer Wildarten). Zentralbl Veterinärmed Suppl 10:1–180
Hofmann RR (1973) The ruminant stomach. East African Literature Bureau, Nairobi
Hofmann RR, Streich WJ, Fickel J, Hummel J, Clauss M (2008) Convergent evolution in feeding types: salivary gland mass differences in wild ruminant species. J Morphol 269:240–257
Hummel J, Clauss M, Zimmermann W, Johanson K, Norgaard C, Pfeffer E (2005) Fluid and particle retention in captive okapi (Okapia johnstoni). Comp Biochem Physiol A 140:436–444
Hummel J, Steuer P, Südekum KH, Hammer S, Hammer C, Streich WJ, Clauss M (2008a) Fluid and particle retention in the digestive tract of the addax antelope (Addax nasomaculatus)—adaptations of a grazing desert ruminant. Comp Biochem Physiol A 149:142–149
Hummel J, Südekum KH, Bayer D, Ortmann S, Hatt JM, Clauss M (2008b) Physical characteristics of reticuloruminal contents of cattle in relation to forage type and time after feeding. J Anim Physiol Anim Nutr (in press). DOI 10.1111/j.1439-0396.2008.00806.x
Jiang Z, Hudson RJ (1996) Digestive responses of wapiti to seasonal forages. Acta Theriol 41:415–425
Kaske M, Midasch A, Rehage J (1994) Sonographic investigation of reticular contractions in healthy sheep, cows, and goats and in cows with traumatic reticulo-peritonitis. J Vet Med Ser A 41:748–756
Kirberger RM (1995) Imaging artifacts in diagnostic ultrasound—a review. Vet Radiol Ultrasound 36:297–306
Kovács PL, Südekum KH, Stangassinger M (1997) Effects of intake level of a mixed diet on chewing activity and on particle size of ruminated boli, ruminal digesta fractions and faeces of steers. Reprod Nutr Dev 37:517–528
Lechner-Doll M, Kaske M, Engelhardt Wv (1991) Factors affecting the mean retention time of particles in the forestomach of ruminants and camelids. In: Tsuda T, Sasaki Y, Kawashima R (eds) Physiological aspects of digestion and metabolism in ruminants. Academic, San Diego, pp 455–482
Meyer RM, Bartley EE (1971) Bloat in cattle. XV. The relation of viscosity and cell-free polysaccharide content of rumen fluid to feedlot bloat. J Anim Sci 33:1018–1020
Meyer RM, Bartley EE (1972) Bloat in cattle. XVI. Development and application of techniques for selecting drugs to prevent feedlot bloat. J Anim Sci 34:234–240
Nygren K, Hofmann RR (1990) Seasonal variations of food particle size in moose. Alces 26:44–50
Pennick DG (1995) Imaging artifacts in ultrasound. In: Nyland TG, Mattoon JD (eds) Veterinary diagnostic ultrasound. Saunders, Philadelphia, pp 19–29
Renecker LA, Hudson RJ (1990) Digestive kinetics of moose, wapiti and cattle. Anim Prod 50:51–61
Robbins CT, Spalinger DE, Van Hoven W (1995) Adaptations of ruminants to browse and grass diets: are anatomical-based browser-grazer interpretations valid? Oecologia 103:208–213
Sakauchi R, Hoshino S (1981) Effects of monensin on ruminal fluid viscosity, pH, volatile fatty acids and ammonia levels, and microbial activity and population in healthy and bloated feedlot steers. J Anim Physiol Anim Nutr 46:21–33
Schwarm A, Ortmann S, Wolf C, Clauss M (2008) Excretion patterns of fluid and different sized particle passage markers in banteng (Bos javanicus) and pygmy hippopotamus (Hexaprotodon liberiensis): Two functionally different foregut fermenters. Comparative Biochemistry and Physiology A (in press). DOI 10.1016/j.cbpa.2008.02.022
Seo S, Lanzas C, Tedeschi LO, Fox DG (2007) Development of a mechanistic model to represent the dynamics of liquid flow out of the rumen and to predict the rate of passage of liquid in dairy cows. J Dairy Sci 90:840–855
Stanford K, Wang Y, Berg BP, Majak W, McCartney DH, Baron V, McAllister TA (2001) Effects of alcohol ethoxylate and pluronic detergents on the development of pasture bloat in cattle and sheep. J Dairy Sci 84:167–176
Warner RG, Flatt WP, Loosli JK (1956) Dietary factors influencing the development of the ruminant stomach. Agic Food Chem 4:788–792
Welch JG (1982) Rumination, particle size and passage from the rumen. J Anim Sci 54:885–894
Acknowledgement
We thank Prof. Dr. Dr. h.c. Ueli Braun for his support, Jessica Gull for the initial spark for this investigation, Michael Bless for the handling of the domestic cows, Andreas Peemöller for the habituation, preparation and care of the moose used in this study, and Marianne Mathys for the graphics design. This investigation was in accord with current Swiss animal welfare laws.
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Tschuor, A., Clauss, M. Investigations on the stratification of forestomach contents in ruminants: an ultrasonographic approach. Eur J Wildl Res 54, 627–633 (2008). https://doi.org/10.1007/s10344-008-0188-5
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DOI: https://doi.org/10.1007/s10344-008-0188-5