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Prelog and anti-Prelog stereoselectivity of two ketoreductases from Candida glabrata

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Abstract

Two ketoreductases from Candida glabrata were used for the asymmetric reduction of prochiral substituted acetophenones displayed different enantiopreference toward para-, meta-substituted and ortho-halogen substituted acetophenones with excellent enantioselectivity. Homology modeling and docking analysis were in conformity with this interested enantiopreference obtained from experimental tests. The reduction of a series of other substituted aryl ketones was also investigated using the two ketoreductases.

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Acknowledgments

This work was financially supported by National Scientific Major Program (2010ZX09301-012) and Shenyang Municipal Scientific and Technology Research Fund (F11-243-1-00).

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Authors and Affiliations

  1. School of Life Sciences & Biopharmaceutical Sciences, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China

    Ping Liang, Bin Qin, Mao Mu, Xin Zhang & Song You

  2. School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China

    Xian Jia

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  1. Ping Liang
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  2. Bin Qin
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  3. Mao Mu
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  4. Xin Zhang
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  5. Xian Jia
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  6. Song You
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Corresponding author

Correspondence to Song You.

Additional information

Ping Liang and Bin Qin contributed equally to this study.

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Liang, P., Qin, B., Mu, M. et al. Prelog and anti-Prelog stereoselectivity of two ketoreductases from Candida glabrata . Biotechnol Lett 35, 1469–1473 (2013). https://doi.org/10.1007/s10529-013-1228-0

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  • DOI: https://doi.org/10.1007/s10529-013-1228-0

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