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Using genetic diversity and mating system parameters estimated from genetic markers to determine strategies for the conservation of Araucaria angustifolia (Bert.) O. Kuntze (Araucariaceae)

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Abstract

In order to understand the impacts of forest fragmentation on Araucaria angustifolia populations, we evaluated the genetic diversity and mating system using SSR markers and open-pollinated seeds from four populations of varying sizes and spatial isolation, in and around one of the best-conserved Araucaria Forest remnants in Southern Brazil. The four population types of A. angustifolia include: (1) a continuous forest; (2) a physically isolated cluster located 2 km from the continuous forest; (3) an open population in a field located between the cluster and continuous forest; and (4) a fragment on a private property located 5 km from the cluster. Approximately 28 seeds were collected from ten reproductive trees in each population. We found higher amounts of alleles (113) and exclusive alleles (25) in the continuous forest than in the other populations. The multilocus paternity correlation was significantly higher and effective number of pollen donors was significantly lower in the private population, decreasing the diversity and consequently the variance effective size of families sampled from that population. However, despite its isolation from the other studied fragments, the private population had the second highest number of alleles as well as unique alleles from the other populations. Therefore, strategies for A. angustifolia conservation should focus not only on larger populations, such as those found in protected areas, but also include smaller and isolated fragments on private properties as these populations are able to maintain high levels of genetic diversity and functional connectivity between isolated stands across a landscape.

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Acknowledgments

The authors acknowledge the financial support provided by CNPq and CAPES as research fellowships to Luciano Medina-Macedo, Juliana Zanetti Ribeiro, Carlos Ricardo Soccol and Alexandre Magno Sebbenn. We also acknowledge the Brazilian Agriculture Research Corporation (EMBRAPA) for logistical and financial support and UFPR and UTFPR for providing the necessary infrastructure. Finally, we thank Dr. Evelyn Nimmo for editing the manuscript.

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Authors and Affiliations

  1. UTFPR - Federal University of Technology - Paraná, Ponta Grossa, PR, CEP 84016-210, Brazil

    Luciano Medina-Macedo & Juliana Vitoria Messias Bittencourt

  2. EMBRAPA Forestry - Brazilian Agriculture Research Corporation, Estrada da Ribeira km 111, CP 319, Colombo, PR, CEP 83411-000, Brazil

    André Eduardo Biscaia de Lacerda

  3. São Paulo Forestry Institute, CP 1322, São Paulo, SP, CEP 01059-970, Brazil

    Alexandre Magno Sebbenn

  4. UFPR - Federal University of Paraná, CP 19011, Curitiba, PR, CEP 81531-990, Brazil

    Juliana Zanetti Ribeiro & Carlos Ricardo Soccol

Authors
  1. Luciano Medina-Macedo
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  2. André Eduardo Biscaia de Lacerda
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  3. Alexandre Magno Sebbenn
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  4. Juliana Zanetti Ribeiro
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  5. Carlos Ricardo Soccol
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  6. Juliana Vitoria Messias Bittencourt
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Correspondence to André Eduardo Biscaia de Lacerda.

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Medina-Macedo, L., de Lacerda, A.E.B., Sebbenn, A.M. et al. Using genetic diversity and mating system parameters estimated from genetic markers to determine strategies for the conservation of Araucaria angustifolia (Bert.) O. Kuntze (Araucariaceae). Conserv Genet 17, 413–423 (2016). https://doi.org/10.1007/s10592-015-0793-2

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