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Isolation, expansion and characterisation of mesenchymal stem cells from human bone marrow, adipose tissue, umbilical cord blood and matrix: a comparative study

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Abstract

The multipotent and immunosuppressive capacities of mesenchymal stem cells (MSCs) attract several scientists worldwide towards translational research focusing on treatment of diseases including liver failure. Though MSC’s have been isolated from different sources, researchers do not concur on the best source for expansion and clinical translation. In this study, we have compared the isolation, proliferation and expansion of MSCs from umbilical cord blood (UCB), Wharton’s Jelly (WJ), bone marrow (BM) and adipose tissue (AT). MSCs were isolated by density gradient separation from UCB, BM and AT and by both enzymatic and explant method for WJ. The MSCs are characterized by their ability to adhere to plastic, expression of positive (CD105, CD73, CD90, CD29, CD44) and negative (CD45, CD14, CD34) markers by flow cytometry and also by their in vitro adipogenic, osteogenic and chondrogenic differentiation. This comprehensive study clearly shows that WJ is better than UCB both in terms of rapidity, yield and ease of procedure. AT and BM are autologous sources for MSC’s but the specimen collection involves cumbersome and painful procedures and an invasive approach. However being autologous, they are safe and probable candidates for therapeutic future applications.

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Acknowledgments

This study is the preliminary part of the project entitled “Hepatic progenitor cells: isolation from various sources, characterization, expansion and transplantation” funded by Indian Council of Medical Research, New Delhi.

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Authors and Affiliations

  1. Centre for Advanced Research (Stem Cells), Government Stanley Hospital, Chennai, 600 001, Tamilnadu, India

    R. Secunda, Rosy Vennila, A. M. Mohanashankar, M. Rajasundari, S. Jeswanth & R. Surendran

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  1. R. Secunda
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  2. Rosy Vennila
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  3. A. M. Mohanashankar
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  4. M. Rajasundari
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  5. S. Jeswanth
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Correspondence to Rosy Vennila.

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Secunda, R., Vennila, R., Mohanashankar, A.M. et al. Isolation, expansion and characterisation of mesenchymal stem cells from human bone marrow, adipose tissue, umbilical cord blood and matrix: a comparative study. Cytotechnology 67, 793–807 (2015). https://doi.org/10.1007/s10616-014-9718-z

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