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Magnetic composites from minerals: study of the iron phases in clay and diatomite using Mössbauer spectroscopy, magnetic measurements and XRD

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Abstract

Magnetic particles as matrix for enzyme immobilization have been used and due to the enzymatic derivative can be easily removed from the reaction mixture by a magnetic field. This work presents a study about the synthesis and characterization of iron phases into magnetic montmorillonite clay (mMMT) and magnetic diatomaceous earth (mDE) by 57Fe Mössbauer spectroscopy (MS), magnetic measurements and X-ray diffraction (XRD). Also these magnetic materials were assessed as matrices for the immobilization of invertase via covalent binding. Mössbauer spectra of the magnetic composites performed at 4.2 K showed a mixture of magnetite and maghemite about equal proportion in the mMMT, and a pure magnetite phase in the sample mDE. These results were verified using XRD. The residual specific activity of the immobilized invertase on mMMT and mDE were 83 % and 92.5 %, respectively. Thus, both magnetic composites showed to be promising matrices for covalent immobilization of invertase.

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Authors and Affiliations

  1. Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Cidade Universitária, PE CEP 50670-901, Recife, PE, Brazil

    M. Cabrera, J. C. Maciel & L. B. de Carvalho Jr

  2. Instituto de Investigaciones para la Industria Química, Universidad Nacional, de Salta - CONICET, Buenos Aires N° 177, 4400, Salta, Argentina

    M. Cabrera & F. Soria

  3. Centro Brasileiro de Pesquisas Físicas, Urca, 22290-180, Rio de Janeiro, RJ, Brazil

    J. Quispe-Marcatoma, B. Pandey & E. Baggio-Saitovitch

  4. Department of Applied Science, Symbiosis Institute of Technology, Mulsi, Pune, 412 115, India

    B. Pandey

  5. Universidade Federal do Vale de São Francisco, Campus Petrolina, 56304-917, Petrolina, PE, Brazil

    D. F. M. Neri

  6. Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Cidade Universitária, 50670-901, Recife, PE, Brazil

    L. B. de Carvalho Jr

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  1. M. Cabrera
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  2. J. C. Maciel
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  3. J. Quispe-Marcatoma
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  4. B. Pandey
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  5. D. F. M. Neri
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  6. F. Soria
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  7. E. Baggio-Saitovitch
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  8. L. B. de Carvalho Jr
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Corresponding author

Correspondence to L. B. de Carvalho Jr.

Additional information

Proceedings of the Thirteenth Latin American Conference on the Applications of the Mössbauer Effect, (LACAME 2012), Medellín, Columbia, 11 16 November 2012.

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Cabrera, M., Maciel, J.C., Quispe-Marcatoma, J. et al. Magnetic composites from minerals: study of the iron phases in clay and diatomite using Mössbauer spectroscopy, magnetic measurements and XRD. Hyperfine Interact 224, 197–204 (2014). https://doi.org/10.1007/s10751-013-0858-x

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  • DOI: https://doi.org/10.1007/s10751-013-0858-x

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