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The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles

  • Gonadal Physiology and Disease
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

This study was designed to investigate the effect of alpha-lipoic acid (ALA) on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and developmental competence of cultured pre-antral follicles derived from mouse ovarian tissue.

Methods

Pre-antral follicles were isolated from immature mouse ovaries and were cultured in α- minimal essential medium supplemented with different concentrations (0, 50, 100, 250 and 500 uM) of ALA. Follicular growth, oocyte maturation and embryo development were evaluated. Separately, ROS and TAC were measured after 0, 24, 48, 72 and 96 h of culture with spectrofluorometery and ferric reducing/antioxidant power (FRAP) assay, respectively.

Results

In the presence of 100 uM ALA, developmental rates of follicles, oocytes and embryos were significantly higher than other groups (p < 0.05). At 96 h after culture, a decrease in ROS and an increase in TAC were observed in ALA group compared to control group (p < 0.05).

Conclusion

ALA (100 uM) improves the in vitro development of follicles. This effect may be mediated by decreasing ROS concentration and increasing follicular TAC level during the culture period.‎‎‎

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Acknowledgements

This work was conducted and funded by the biology school of Damghan University, Damghan, Iran.

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Authors and Affiliations

  1. School of Biology, Damghan University, Damghan, Iran

    Ali Talebi, Saeed Zavareh, Maryam Hajighasem Kashani & Taghi Lashgarbluki

  2. School of Veterinary medicine, Razi University, Kermanshah, Iran

    Isaac Karimi

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  1. Ali Talebi
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  2. Saeed Zavareh
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  3. Maryam Hajighasem Kashani
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Correspondence to Saeed Zavareh.

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Alpha lipoic acid improved the developmental competence of preantral follicles in mice

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Talebi, A., Zavareh, S., Kashani, M.H. et al. The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles. J Assist Reprod Genet 29, 175–183 (2012). https://doi.org/10.1007/s10815-011-9706-6

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