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Cross-Reactive Antibodies to Target Proteins are Dependent upon Oligomannose Glycosylated Epitopes in HTLV-1 Associated Neurological Disease

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Abstract

Our lab recently identified a cross-reactive antibody response between human T-lymphotropic virus type-1-p24-(gag) (HTLV-1-p24-(gag)) and peroxiredoxin-1 (PrX-1) as potentially contributing to the pathogenesis of HTLV-1 associated neurological disease via molecular mimicry. These targets proteins were glycosylated, yet the glycan side chains immunoreactive with the immunoglobulins were unknown. Using a combination of lectin isolation and serial enzymatic deglycosylation of glycoproteins, we determined that the immunoreactive epitopes contained branched oligomannose side chains. These data suggest that post-translational glycosylation specifically related to oligomannose immunoreactivity to both the infecting and host antigens may contribute to molecular mimicry and be important in the pathogenesis of HTLV-1 associated neurological disease.

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Acknowledgements

This work is based upon work supported by the Office of Research and Development, Medical Research Service, Department of Veterans Affairs. This study was funded by a VA Merit Review Award (to MCL) and the Multiple Sclerosis Research Fund of the University of Tennessee Health Science Center.

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Authors and Affiliations

  1. Research Service, Veterans Administration Medical Center, University of Tennessee Health Science Center, 1030 Jefferson Avenue, Memphis, TN, 38104, USA

    Sangmin Lee, Yoojin Shin & Michael C. Levin

  2. Department of Neurology, University of Tennessee Health Science Center, Link Building Room 415, 855 Monroe Avenue, Memphis, TN, 38163, USA

    Sangmin Lee, Yoojin Shin & Michael C. Levin

  3. Instituto de Medicina Tropical, Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru

    Daniel Clark & Eduardo Gotuzzo

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  1. Sangmin Lee
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Correspondence to Michael C. Levin.

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Lee, S., Shin, Y., Clark, D. et al. Cross-Reactive Antibodies to Target Proteins are Dependent upon Oligomannose Glycosylated Epitopes in HTLV-1 Associated Neurological Disease. J Clin Immunol 32, 736–745 (2012). https://doi.org/10.1007/s10875-012-9652-9

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