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Microcalorimetric study of the growth of Enterococcus faecalis, Pseudomonas aeruginosa and their mixtures in an enriched culture medium

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Abstract

Microcalorimetry is a highly sensitive experimental technique that allows to determine the energy released by any process or transformation. In the field of medicine, it is interesting for investigations of microbial processes. The interaction relationship between Enterococcus faecalis and Pseudomonas aeruginosa was researched using a Tian–Calvet calorimeter equipped with two stainless steel cells (reference and experimental). Three samples from both bacteria were prepared in the following proportions: 20 + 80 % (0.2 mL E. faecalis + 0.8 mL P. aeruginosa), 50 + 50 % (0.5 mL E. faecalis + 0.5 mL P. aeruginosa) and 80 + 20 % (0.8 mL E. faecalis + 0.2 mL P. aeruginosa). Experiments were carried out at a concentration of 103 CFU mL−1 and a constant temperature of 309.65 K. Recording the heat voltage difference versus time, the growth curves for E. faecalis, P. aeruginosa and their mixtures were obtained. The differences in shape of curves of single microorganisms and their mixtures were compared. Also, the thermokinetic parameters of single microorganisms and their mixtures (growth constant, generation time, detection time and amount of heat released) were calculated.

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Acknowledgements

We thank María Perfecta Salgado Gonzalez and Sofia Baz Rodríguez for their collaboration with the technical measures. We are also thankful for the financial support provided by the Projects EM 2012/141, CN 2012/285, and “Agrupación Estratégica de Biomedicina (INBIOMED)” by “Xunta de Galicia” and the Project FIS 2011-23322 funded by Ministry of Science and Innovation of Spain. All these projects are co-financed with FEDER funds.

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Authors and Affiliations

  1. Department of Applied Physics, University of Vigo, Lagoas Marcosende, 36310, Vigo, Spain

    C. Vazquez, M. M. Mato & J. L. Legido

  2. Hospital Complex University of Vigo, Pharmacy Service, Pizarro no 22, 36204, Vigo, Spain

    N. Lago & L. Esarte

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  1. C. Vazquez
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  2. N. Lago
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  4. J. L. Legido
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Correspondence to C. Vazquez.

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Vazquez, C., Lago, N., Mato, M.M. et al. Microcalorimetric study of the growth of Enterococcus faecalis, Pseudomonas aeruginosa and their mixtures in an enriched culture medium. J Therm Anal Calorim 121, 463–468 (2015). https://doi.org/10.1007/s10973-015-4606-0

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