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Sera of Peruvians with fever of unknown origins include viral nucleic acids from non-vertebrate hosts

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Abstract

Serum samples collected from 88 Peruvians with unexplained fever were analyzed for viral sequences using metagenomics. Nucleic acids of anelloviruses, pegivirus A (GBV-C), HIV, Dengue virus, and Oropouche virus were detected. We also characterized from two sera the RNA genomes of new species of partitivirus and dicistrovirus belonging to viral families known to infect fungi or arthropod, respectively. Genomic DNA of a putative fungal cellular host could be PCR amplified from the partitivirus-containing serum sample. The detection in human serum of nucleic acids from viral families not known to infect vertebrates may indicate contamination during sample collection and aliquoting or human infection by their presumed cellular host, here a fungus. The role, if any, of the non-vertebrate infecting viruses detected in serum in inducing fever is unknown.

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Acknowledgements

We acknowledge the support from Blood Systems Research Institute. We thanks Dr. Paul D. N. Hebert and Sean Prosser from the Center for Biodiversity Genomics at the University of Guelph, Ontario, Canada, with crucial assistance selecting the arthropod specific PCR primers.

Funding

Funding was provided by National Heart, Lung, and Blood Institute (Grant No. R01 HL105770).

Author information

Authors and Affiliations

  1. Blood Systems Research Institute, San Francisco, CA, 94118, USA

    Tung Gia Phan, Mohammadreza Sadeghi, Eda Altan, Xutao Deng & Eric Delwart

  2. Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA, 94118, USA

    Tung Gia Phan, Mohammadreza Sadeghi, Eda Altan & Eric Delwart

  3. School of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru

    Juana del Valle Mendoza

  4. Instituto de Investigación Nutricional, Lima, Peru

    Juana del Valle Mendoza

  5. Department of Virology, University of Helsinki, Helsinki, Finland

    Mohammadreza Sadeghi

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  1. Tung Gia Phan
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Contributions

TP, JM, and ED: designed the study; TP, MS, EA, and XD: performed experiments; TP, JM, and ED: wrote the manuscript; JM: provided clinical information and samples.

Corresponding author

Correspondence to Eric Delwart.

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Research involving Human Participants and/or Animals

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Edited by Detlev H. Kruger.

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Phan, T.G., del Valle Mendoza, J., Sadeghi, M. et al. Sera of Peruvians with fever of unknown origins include viral nucleic acids from non-vertebrate hosts. Virus Genes 54, 33–40 (2018). https://doi.org/10.1007/s11262-017-1514-3

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