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Antioxidant, anti-inflammatory, and anti-apoptotic effects of crocin against doxorubicin-induced myocardial toxicity in rats

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Abstract

Doxorubicin (DOX) is a well-known chemotherapeutic drug for most malignancies including breast cancer and leukemia whilst the usage of DOX is limited owing to its cardiotoxicity. In the present study, we aimed to investigate the effects of crocin on doxorubicin-induced cardiotoxicity in rats. Forty rats were randomly divided into four groups: (a) control [received normal saline as a dose of 1 ml/kg by intraperitoneal injection (ip) for 15 days], (b) crocin (received crocin as a dose of 40 mg/kg/24h by ip for 15 days), (c) DOX (received DOX as a dose of 2 mg/kg/48h by ip in six injection, cumulative dose 12 mg/kg), and (d) DOX+crocin (received DOX as a dose of 2 mg/kg/48h by ip in six injection, and crocin as a dose of 40 mg/kg/24h i.p for 15 days). As compared to the controls, the results showed that DOX administration caused significant increases in lipid indices [triglyseride (TG), low-dencity lipoproteins (LDL) (p<0.001), and very low-dencity lipoproteins (VLDL) (p<0.005)], oxidative stress parameters [malondialdehyde (MDA) and total oxidant status (TOS) (p<0.001)] and cardiac markers [creatine kinase-muscle/brain (CK-MB) and cardiac troponin I (cTnI) (p<0.001)]. Besides, significant decreases in antioxidant defense systems [glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and total antioxidant status (TAS) (p<0.001)] were observed. The present study also demonstrated that co-administration of crocin with DOX significantly ameliorated the lipid profile (p<0.005), cardiac markers (p<0.005), and oxidative stress indices (p<0.001) as compared to DOX group. Histopathologically, significant increase in the mean histopathological damage score (MHDS) was found in the DOX group as compared to the controls (p<0.001). In contrast, the administration of crocin with DOX alleviated MHDS in myocardium (p<0.001). Taken together, our results reveal that crocin might be a cardioprotective agent in DOX-treated patients for cancer.

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The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

The research was supported by Karabuk University Scientifict Research Fund (TYL-2020-2298).

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Authors and Affiliations

  1. Department of Medical Biochemistry, Faculty of Medicine, Karabuk University, Karabuk, Turkey

    Sara Asaad Abdulkareem Aljumaily, Yasemin Bicer & Eyup Altinoz

  2. Department of Physiology, Faculty of Medicine, Karabuk University, Karabuk, Turkey

    Mehmet Demir

  3. Department of Histology and Embryology, Faculty of Medicine, Mugla Sıtkı Kocman University, Mugla, Turkey

    Hulya Elbe & Gurkan Yigitturk

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  1. Sara Asaad Abdulkareem Aljumaily
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Sara Asaad ABDULKAREEM ALJUMAILY and Yasemin Bicer studied biochemical analysis, Mehmet Demir designed the study and collected the tissues, Hulya Elbe and Gurkan Yigitturk performed the histological examination of the hearth tissues, and Eyup Altınoz designed the study and calculated the biochemical results, and was a major contributor in writing the manuscript.

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Correspondence to Eyup Altinoz.

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The study was approved by the Experimental Animals Ethics Committee of Zonguldak Bulent Ecevit University, Faculty of Medicine (Protocol No: 2020/04).

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Abdulkareem Aljumaily, .A., Demir, M., Elbe, H. et al. Antioxidant, anti-inflammatory, and anti-apoptotic effects of crocin against doxorubicin-induced myocardial toxicity in rats. Environ Sci Pollut Res 28, 65802–65813 (2021). https://doi.org/10.1007/s11356-021-15409-w

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