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Effects of Boron on Structure and Antioxidative Activities of Spleen in Rats

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Abstract

In order to determine the relationship between boron and development of the spleen, especially in the promoting biological effects, we examined the effects of different levels of boron on weight, organ index, microstructure, and antioxidative activities of the spleen in rats. Sprague-Dawley (SD) rats were selected and treated with different concentrations of boron, and then, the organs were resected and weighed. One half of the tissue was fixed and embedded in paraffin to observe tissue structure changes. The other half of the tissue was homogenated for determining the antioxidant activities. The results showed that 40 mg/L of boron could increase weight, organ indexes, and antioxidant capacity of spleens and improve the spleen tissue structure, while the boron concentration above 80 mg/L could decrease weight, organ indexes, and antioxidant capacity of spleens and damage the spleen tissue structure. The higher the concentration, the more serious the damage was. Especially at the concentration of 640 mg/L, it could significantly inhibit the development of the spleen and even exhibit toxic effect. Hence, low boron concentration played a protective role in the development of the spleen, while high boron concentration could damage the organs and even produce toxic effect.

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References

  1. Nielsen FH (2002) The nutritional importance and pharmacological potential of boron for higher animals and human. In: Goldbach HE et al (eds) Boron in plant and animal nutrition. Kluwer Academic/Plenum, New York, pp 37–49

    Chapter  Google Scholar 

  2. Haro Durand LA, Mesones RV, Nielsen FH, Gorustovich AA (2010) Histomorphometric and microchemical characterization of maturing dental enamel in rats fed a boron-deficient diet. Biol Trace Elem Res 135:242–252

    Article  CAS  PubMed  Google Scholar 

  3. Cheng JY, Peng KM, Jin EH, Zhang Y, Liu Y, Zhang NB, Song H, Liu HZ, Tang ZL (2011) Effect of additional boron on tibias of African ostrich chicks. Biol Trace Elem Res 144:538–549

    Article  CAS  PubMed  Google Scholar 

  4. Ying XZ, Cheng SW, Wang W, Lin ZQ, Chen QY, Zhang W, Kou DQ, Shen Y, Cheng XJ, Rompis FA, Peng L, Zhu Lu C (2011) Effect of boron on osteogenic differentiation of human bone marrow stromal cells. Biol Trace Elem Res 144:306–315

    Article  CAS  PubMed  Google Scholar 

  5. Geyikoğlu F, Türkez H (2007) Acute toxicity of boric acid on energy metabolism of the breast muscle in broiler chickens. Biologia, Bratislava 62(1):112–117

    Article  Google Scholar 

  6. Kloppmann W, Bianchini G, Charalambides A, Dotsika E, Guerrot C, Klose P, Marei A, Pennisi M, Vengosh A, Voutsa D (2005) Boron contamination of Mediterranean groundwater resources: extent, sources and pathways elucidated by environmental isotopes. Geophys Res Abstr 7:10162

    Google Scholar 

  7. Armstrong TA, Spears JW (2003) Effects of boron supplementation of pig diets on the production of tumor necrosis factor-α and interferon-γ. J Anim Sci 81(10):2552–2561

    CAS  PubMed  Google Scholar 

  8. Armstrong TA, Spears JW, Lloyd KE (2001) Inflammatory response, growth, and thyroid hormone concentrations are affected by long-term boron supplementation in gilts. J Anim Sci 79(6):1549–1556

    CAS  PubMed  Google Scholar 

  9. Müezzinoğlu T, Korkmaz M, Nese N et al (2011) Prevalence of prostate cancer in high boron-exposed population: a community-based study. Biol Trace Elem Res 144:49–57

    Article  PubMed  Google Scholar 

  10. O’Sullivan K, Taylor M (1983) Chronic boric acid poisoning in infants. Arch Dis Child 58:737–749

    Article  PubMed Central  PubMed  Google Scholar 

  11. Litovitz TL, Klein-Schwartz W, Oderda GM, Schmitz BF (1988) Clinical manifestations of toxicity in a series of 784 boric acid ingestions. Am J Emerg Med 6:209–213

    Article  CAS  PubMed  Google Scholar 

  12. Devirian TA, Volpe SL (2003) The physiological effects of dietary boron. Crit Rev Food Sci Nutr 43:219–231

    Article  CAS  PubMed  Google Scholar 

  13. Fail PA, Chapin RE, Price CJ, Heindel JJ (1998) General, reproductive, developmental and endocrine toxicity of boronated compounds. Reprod Toxicol 12(1):1–18

    Article  CAS  PubMed  Google Scholar 

  14. Hubbard SA, Sullivan FM (1996) Toxicological effects of inorganic boron compounds in animals: a review of the literature. J Trace Elem Exp Med 9:165–173

    Article  CAS  Google Scholar 

  15. Gu YF, Li SH, Wang J, Shang CF, Chen HL (2007) Effects of boron poisoning on thymus development in Gushi chickens. Indian Vet J 84(6):584–586

    CAS  Google Scholar 

  16. Hunt CD (1998) One possible role of dietary boron in higher animals and humans. Biol Trace Elem Res 66(1–3):205–225

    Article  CAS  PubMed  Google Scholar 

  17. Hunt CD, Idso JP (1999) Dietary boron as a physiological regulator of the normal inflammatory response: a review and current research progress. J Trace Elem Exp Med 12:221–233

    Article  CAS  Google Scholar 

  18. Bai Y, Hunt CD, Newman SM (1997) Dietary boron increases serum antibody (IgG and IgM) concentrations in rats immunized with human typhoid vaccine. Proc North Dakota Acad Sci 51:181

    Google Scholar 

  19. Bai Y, Hunt CD (1995) Dietary boron alleviates adjuvant-induced arthritis (AIA) in rats. FASEB J 9:A576

    Google Scholar 

  20. Ince S, Kucukkurt I, Cigerci IH, Fatih Fidan A, Eryavuz A (2010) The effects of dietary boric acid and borax supplementation on lipid peroxidation, antioxidant activity, and DNA damage in rats. J Trace Elem Med Biol 24(3):161–164

    Article  CAS  PubMed  Google Scholar 

  21. Ince S, Keles H, Erdogan M, Hazman O, Kucukkurt I (2012) Protective effect of boric acid against carbon tetrachloride-induced hepatotoxicity in mice. Drug Chem Toxicol 35(3):285–292

    Article  CAS  PubMed  Google Scholar 

  22. Mebius RE, Kraal G (2005) Structure and function of the spleen. Nat Rev Immunol 5:606–616

    Article  CAS  PubMed  Google Scholar 

  23. Feng BM, Li X, Li SH et al (2009) Effect of drinking boron on blood composition in rats. J Hyg Res 38(4):485–488 (in Chinese)

    CAS  Google Scholar 

  24. Kurtoğlu F, Kurtoğlu V, Celik I, Kececi T, Nizamlioğlu M (2005) Effects of dietary boron supplementation on some biochemical parameters, peripheral blood lymphocytes, splenic plasma cells and bone characteristics of broiler chicks given diets with adequate or inadequate cholecalciferol (vitamin D3) content. Br Poult Sci 46(1):87–96

    Article  PubMed  Google Scholar 

  25. Hunt CD (2003) Dietary boron: an overview of the evidence for its role in immune function. J Trace Elem Exp Med 16:291–306

    Article  CAS  Google Scholar 

  26. Li SH, Fan GL, Gu YF, Wang J, Peng KM, Jin GM, Liu DY, Chen HL (2009) Effect of drinking boron supplemented on the development of center immune organs in Gushi chickens. J Northwest A F Univ (Nat Sci Ed) 2(37):52–58 (in Chinese)

    Google Scholar 

  27. Li SH, Gu YF, Fan GL, Wang J, Peng KM, Jin GM, Chen HL, Shang CF (2008) Effect of different level drinking boron supplemented on the development of immune organs in Gushi chickens. Chin Soc Immunol 1(24):47–48 (in Chinese)

    Google Scholar 

  28. Li SH, Zhu HG, Wang J, Jin GM, Gu YF, Liu DY (2009) Effect of environmental estrogen boron on microstructure of thymus in rats. J Anhui Sci Technol Univ 23(6):1–5 (in Chinese)

    Google Scholar 

  29. Wang J, Li SH, Jin GM, Shang CF, Chen HL, Gu YF (2007) Effect of drinking-boron on jejunum development of animal. Stud Trace Elem Health 24(2):1–4 (in Chinese)

    Google Scholar 

  30. Hunt CD, Herbel JL, Idso JP (1994) Dietary boron modifies the effects of vitamin D3 nutrition on indices of energy substrate utilization and mineral metabolism in the chick. J Bone Miner Res 9(2):171–182

    Article  CAS  PubMed  Google Scholar 

  31. Pahl MV, Culver BD, Vaziri ND (2005) Boron and the kidney review. J Ren Nutr 15(4):362–370

    Article  PubMed  Google Scholar 

  32. Wang K (1996) Trace elements in life sciences, 2nd edn. Chinese Metrology, Beijing, pp 377–402

    Google Scholar 

  33. Wang J, Gu YF, Li SH, Shang CF, Chen HL (2005) Effect of boron on development of jejunum in Gushi chickens. Chin J Vet Sci 25(5):514–517 (in Chinese)

    Google Scholar 

  34. Bakirdere S, Orenay S, Korkmaz M (2010) Effect of boron on human health. Open Miner Process J 3:54–59

    CAS  Google Scholar 

  35. Kucukkurt I, Akbel E, Karabag F, Ince S (2013) The effects of dietary boron compounds in supplemented diet on hormonal activity and some biochemical parameters in rats. Toxicol Ind Health. doi:10.1177/0748233712469648

    Google Scholar 

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Acknowledgements

This work was supported by Key Projects of Scientific and Technological Research of Education Ministry, China (210095); Natural Science Fund of Anhui, China (090411001, 1308085MC50, and 1208085QC68); Key Discipline Construction Program of Anhui Science and Technology University, China (AKXK20101-2); and Talents Special Foundation of Anhui Science and Technology University, China (ZRC2013353 and ZRC2013354).

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  1. Anhui Science and Technology University, Fengyang, China

    Qianqian Hu, Shenghe Li, Enmei Qiao, Zhongtao Tang, Erhui Jin, Guangming Jin & Youfang Gu

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  1. Qianqian Hu
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  2. Shenghe Li
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  3. Enmei Qiao
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  4. Zhongtao Tang
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  5. Erhui Jin
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Correspondence to Shenghe Li.

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Hu, Q., Li, S., Qiao, E. et al. Effects of Boron on Structure and Antioxidative Activities of Spleen in Rats. Biol Trace Elem Res 158, 73–80 (2014). https://doi.org/10.1007/s12011-014-9899-5

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  • DOI: https://doi.org/10.1007/s12011-014-9899-5

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