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Dietary Supplementation of Selenium-Enriched Probiotics Enhances Meat Quality of Broiler Chickens (Gallus gallus domesticus) Raised Under High Ambient Temperature

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Abstract

We investigated the effects of selenium-enriched probiotics (SP) on broiler meat quality under high ambient temperature and explore their underlying mechanisms. A total of 200 1-day-old male broiler chicks (Ross 308) were randomly allotted to four treatment groups, each with five replicates, in groups of ten birds. These birds were fed a corn-soybean basal diet (C), a basal diet plus probiotics supplementation (P), a basal diet plus Se supplementation in the form of sodium selenite (SS, 0.30 mg Se/kg), and a basal diet with the addition of selenium-enriched probiotics (SP, 0.30 mg Se/kg). The experiment lasted for 42 days. The birds were sacrificed by cervical dislocation, and the breast muscles were removed for further process. Our results showed that SP diet significantly increased (p < 0.05) the physical (pH, colors, water holding capacity, drip loss, shear force) and sensory characteristics of breast meat. All P, SS, and SP supplementation enhanced the antioxidant system by increasing (p < 0.05) the Se concentrations, glutathione (GSH) levels, activities of glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) whereas decreasing (p < 0.05) malondialdehyde (MDA) levels, with SP being higher than P and SS. Moreover, SP diet significantly upregulated (p < 0.05) the mRNA levels of glutathione peroxidase genes (GPx1, GPx4) while it downregulated heat stress biomarkers such as heat shock protein (HSP) 70 as compared to C, P, and SS diets. In conclusion, our findings suggest that SP may function as beneficial nutritive supplement that is capable of improving meat quality during the summer season.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31272627,31472253), the Research Fund for Doctoral Program of Higher Education in China (20110097110014, 20120097130002), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Authorship

K.H. formulated the research question and designed the research; A.Z.K., S.K., Y.L., M.H., C.P., S.A.N., and F.P. conducted the research. A.Z.K., S.K., Y.L., and K.H. analyzed the data; A.Z.K and K.H. wrote the paper; and K.H. is responsible for the final content. All authors read and approved the final manuscript.

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Authors and Affiliations

  1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 7 Jiangsu Province, 210095, China

    Alam Zeb Khan, Shahnawaz Kumbhar, Yunhuan Liu, Mohammad Hamid, Cuiling Pan, Sonia Agostinho Nido, Fahmida Parveen & Kehe Huang

  2. Institute of Nutritional and Metabolic Disorders of Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing, 210095, China

    Alam Zeb Khan, Shahnawaz Kumbhar, Yunhuan Liu, Mohammad Hamid, Cuiling Pan, Sonia Agostinho Nido & Fahmida Parveen

  3. Gomal College of Veterinary Sciences, Gomal University, Dera Ismail Khan, 29050, Pakistan

    Alam Zeb Khan

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  1. Alam Zeb Khan
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Correspondence to Kehe Huang.

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All protocols for conducting the experimental animals were approved by the “Committee for Care and Use of Experimental Animals” of Nanjing Agriculture University (animal ethical number: SYXK (Su) 2011-0036).

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Khan, A.Z., Kumbhar, S., Liu, Y. et al. Dietary Supplementation of Selenium-Enriched Probiotics Enhances Meat Quality of Broiler Chickens (Gallus gallus domesticus) Raised Under High Ambient Temperature. Biol Trace Elem Res 182, 328–338 (2018). https://doi.org/10.1007/s12011-017-1094-z

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