Abstract
Mastitis is a serious problem in dairy sector and among various aetiological agents, the incidence of staphylococci and streptococci remains high in milking animal. The present study was focused on detection of staphylococci and streptococci in winter season. Milk samples (117) of mastitic animals were tested for presence of staphylococci and streptococci using biochemical and PCR based assays. The testing revealed majority of animals (90.6%) were infected with more than one causative agent. Amongst 117 sample, 109 and 90 comprised of staphylococci and 90 streptococci, respectively. Distribution proportion of S. aureus, S. epidermidis, S. agalactiae, S. uberis and S. dysgalactiae among the mastitic cases was found as 64.9, 7.7, 5.1, 1.7, 48.7, 65.8 and 0.8%, respectively. Streptococci and staphylococci were observed in different combinations and the frequent were S. aureus/S. agalactiae/S. uberis, S. aureus/S. uberis, S. aureus/S. agalactiae and S. agalactiae/S. uberis which were accounted for 23.9, 19.7, 5.9 and 2.6%, respectively. Approximately half of the (52.1%) cases were observed for reoccurrence of mastitis. Reoccurrence of mastitis in winter season among these cases was significantly low as compared to summer (cattle-5 cases; buffaloes-2 cases). In addition, prevalence of S. aureus, S. agalactiae, S. uberis, and S. epidermidis in reoccurring mastitic cases was 73.7, 63.9, 45.9 and 6.6%, respectively. The observations revealed mastitis causing pathogens remains in hidden phase in winter season; however, cannot be neglected. The observation might be helpful in culling or segregation of cows for mastitis reduction programmes.
Similar content being viewed by others
References
Wellenberg GJ, Van der Poel WH, Van Oirschot JT (2002) Viral infections and bovine mastitis: a review. Vet Microbiol 88:27–45
Bar D, Tauer LW, Bennett G, González RN, Hertl JA, Schukken YH et al (2008) The cost of generic clinical mastitis in dairy cows as estimated by using dynamic programming. J Dairy Sci 91:2205–2214
Dua K (2001) Incidence, etiology and estimated loss due to mastitis in India: an update. Ind Dairyman 53:41–48
Watts JL, Salmon SA (1997) Activity of selected antimicrobial agents against strains of Staphylococcus aureus isolated from bovine intra-mammary infections that produce β-lactamase. J Dairy Sci 80:788–791
White DG (1999) Use and misuse of antimicrobials in veterinary medicine. Proceeding of 38th Annual Meeting, Arlington (National Mastitis Council, Inc., Madison), p 9
Bayles KW, Wesson CA, Liou LE, Fox LK, Bohach GA, Trumble WR (1998) Intracellular Staphylococcus aureus escapes the endosome and induces apoptosis in epithelial cells. Infect Immun 66:336–342
Haltia L, Honkanen-Buzalski T, Irina Spiridonova I, Arvi O, Myllys V (2006) A study of bovine mastitis, milking procedures and management practices on 25 Estonian dairy herds. Acta Vet Scand 48(22):1–6
Hawari AD, Fawzi A (2008) Prevalence and distribution of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Jordon. Am J Anim Vet Sci 3:36–39
Lakew M, Tolosa T, Tigre W (2009) Prevalence and major bacterial causes of bovine mastitis in Asella, South Eastern Ethiopia. Trop Anim Health Prod 41:1525–1530
Patil RL, Kumar SP, Shettar VB, Sudhindra, Honnappagol SS (2005) Epidemiology of subclinical mastitis in buffaloes under field conditions of Bidar, Karnataka state (India). Buffalo Bull 24:91–97
Ericsson Unnerstad H, Lindberg A, Persson Waller K, Ekman T, Artursson K, Nilsson-Östa M, et al (2009) Microbial aetiology of acute clinical mastitis and agent-specific risk factors. Vet Microbiol. doi:10.1016/j.vetmic.2008.12.005
Chotar M, Vidova B, Godany A (2006) Development of specific and rapid detection of bacterial pathogen in dairy products by PCR. Folia Microbiol 51:639–646
Riffon RE, Sayasith K, Khalil H, Dubreuil P, Drolet M, Lagace′ J (2001) Development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by PCR. J Clin Microbiol 39:2584–2589
Gillespie BE, Oliver SP (2005) Simultaneous detection of mastitis pathogens, Staphylococcus aureus, Streptococcus uberis, and Streptococcus agalactiae by multiplex real-time polymerase chain reaction. J Dairy Sci 88:3510–3518
Phuektes P, Mansell PD, Browning GF (2001) Multiplex polymerase chain reaction assay for simultaneous detection of Staphylococcus aureus and streptococcal causes of bovine mastitis. J Dairy Sci 84:1140–1148
Kloos WE, Schleifer KH (1986) Genus Staphylococcus. In: Sneath PHA, Mair NS, Sharpe ME and Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 2, Williams & Wilkins, Baltimore, pp 1013–1035
Morot-Bizot SC, Talon R, Leroy S (2004) Development of a multiplex PCR for the identification of Staphylococcus genus and four staphylococcal species isolated from food. J Appl Microbiol 97:1087–1094
Getahun K, Kelay B, Bekana M, Lobago F (2008) Bovine mastitis and antibiotic resistance patterns in Selalle smallholder dairy farms, central Ethiopia. Trop Anim Health Prod 40(4):261–268. doi:10.1007/s11250-007-9090-5
Kerro DO, Tareke F (2003) Bovine mastitis in selected areas of southern Ethiopia. Trop Anim Health Prod 35:197–205. doi:10.1023/A:1023352811751
Sori H, Zerihun A, Abdicho S (2005) Dairy cattle mastitis in and around Sebeta, Ethiopia. Int Appl ResVet Med 3:332–338
Sumathi BR, Veeregowda BM, Gomes AR (2008) Prevalence and antibiogram profile of bacterial isolates from clinical bovine mastitis. Vet World 1:237–238
Ghose B, Sharda R, Chhabra D, Sharma V (2003) Subclinical bacterial mastitis in cows of Malwa region of Madhya Pradesh. Indian Vet Sci J 80:499–501
Shinde SS, Kulkarni GB, Gangane GR, Degloarkar NM (2001) Incidence of mastitis in buffaloes in Parbhani district, Maharashtra. Proc Indian vet Cong, Ludhiana, pp 35–38
Joshi HD, Shrestha HK (1995) Study on the prevalence of clinical mastitis in cattle and buffaloes under different management systems in the western hills of Nepal. Working paper, Lumle Regi Agric Res Cent 4:95–64
Bishop JR, Bodine AB, Janzen JJ (1980) Sensitivities to antibiotics and seasonal occurrence of mastitis pathogens. J Dairy Sci 63:1134–1137
Joshi S, Gokhale S (2006) Status of mastitis as an emerging disease in improved an Periurban dairy farms in India. Ann NY Acad Sci 1081:74–83
Shathele MS (2009) Weather effect on bacterial mastitis in dairy cows. Int J dairy sci 4:57–66
Acknowledgments
This study was supported by Institutional research grant from the National Dairy Research Institute, Karnal, India. Authors are also highly thankful to Rakesh Kumar, Kapil Dev, Anil Kumar and Nanku Singh of Livestock Genome Analysis Laboratory and Milking section of institute for their help in samples collection and support.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sentitula, Yadav, B.R. & Kumar, R. Incidence of Staphylococci and Streptococci During Winter in Mastitic Milk of Sahiwal Cow and Murrah Buffaloes. Indian J Microbiol 52, 153–159 (2012). https://doi.org/10.1007/s12088-011-0207-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12088-011-0207-1