Abstract
Infection with Babesia and Theileria Causes high mortality and economical losses in livestock and has a relatively high prevalence in Iran. In Zabol, animals are susceptible to this disease because of presence of vector ticks, weather conditions and smuggle animal across the border and they cause great damages to the economy and production. However, few studies have been done for differentiation of Theileria and Babesia in sheep in this area. The purpose of the present study was to determine the prevalence of Babesiosis and Theileriosis in sheep in Zabol. A number of 80 animals were randomly selected and their blood samples were examined. The presence of Theileria and Babesia parasites in each sample was determined with PCR and microscopic examination. Of 80 blood samples obtained from sheep, 8 cases (10 %) were positive by microscopic examination where 6 samples were infected with Theileria and 2 samples with Babesia. The result of PCR method determined the prevalence of 66.25 % (53 samples) and 3.75 % (3 samples) for Theileria and Babesia, respectively. The correlation between these two methods (PCR and microscopic examination) was determined with Kappa statistical test. Based on the obtained results, it is concluded that Babesiosis has a low prevalence among the sheep of Zabol. This is the first report in which ovine Babesiosis has been studied in this region using molecular identification techniques.
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Ahmadian A, Ghanbari A, Mir B, Razmjoo E (2009) Intraction effect of drought stress and animal manure on yield componets, essential oil and its compositions on Cumin. Iran J Plant Tehran Univ 40:173–180 (in Persian)
Ahmed J, Yin H, Schnittger L, Jongejan F (2002) Ticks and tick-borne diseases in Asia with special emphasis on China. Parasitol Res 88:51–55
Aktas M, Altay K, Dumanli N (2005) Survey of Theileria parasites of sheep in eastern Turkey using polymerase chain reaction. Small Rumin Res 60:289–293
Altay K, Dumanli N, Holman PJ, Aktas M (2005) Detection of Theileria ovis in naturally infected sheep by nested PCR. Vet Parasitol 127:99–104
Dehkordi ZS, Zakeri S, Nabian S, Bahonar A, Ghasemi F, Noorollahi F, Rahbari S (2010) Molecular and biomorphometrical identification of ovine babesiosis in Iran. Iran J Parasitol 5(4):21
Dumanli N, Aktas M, Cetinkaya B, Cakmak A, Koroglu E, Saki CE et al (2005) Prevalence and distribution of tropical theileriosis in eastern Turkey. Vet Parasitol 127:9–15
Hashemi-Fesharaki R (1997) Tick-borne diseases of sheep and goats and their related vectors in Iran. Parasitologia 39:115–117
Hashemzadeh F, Nabavi L, Seyfabad Shapouri MR, Rahbari S, Azizi F et al (2006) Development of an ELISA technique for the detection of B. ovis and serological survey of the parasite in Khuzestan province, southern Iran. Iran J Univ Shiraz 7(2):15
Heidarpour-Bami M, Khazraiinia P, Haddadzadeh HR, Kazemi B (2010) Identification of Theileria species in sheep in the eastern half of Iran using nested PCR-RFLP and microscopic techniques. Iran J Vet Res 11(3):262–266
Razmi GR, Naghibi A, Aslani MR, Dastjerdi K, Hosseini H (2003) An epidemiological study on Babesia infection in small ruminants in Mashhad suburb, Khorasan province. Iran Small Rumin Res 50(1):39–44
Razmi GR, Eshrati H, Rashtibaf M (2006) Prevalence of Theileria spp. infection in sheep in South Khorasan province, Iran. Vet Parasitol 140:239–243
Schnittger L, Yin H, Qi B, Gubbels MJ, Beyer D, Niemann S, Jongejan F, Ahmed JS (2004) Simultaneously detection and differentiation of Theileria and Babesia parasite infection small ruminants by revers line blotting. Parasitol Res 92:189–196
Seidabadi M, Razmi GhR, Naghibi A (2014) Molecular detection of Babesia spp. in sheep and vector ticks in North Khorasan province, Iran. Iran J Vet Med 8(1):35–39
Shayan P, Rahbari S (2007) Differentiation of sheep Theileria spp. and Babesia spp. by polymerase chain reaction. J Vet Res 62(3):250–260
Shayan P, Ebrahimzadeh E, Tageldin MH, Amininia N, Eckert B (2011) Molecular study of sheep malignant theileriosis at Barka region in the Sultanate of Oman. Iran J Parasitol 6(1):66–72
Soulsby EJL (1982) Helminths, arthropods and protozoa of domesticated animals, 7th edn. Bailliere Tindall, London, pp 706–728
Theodoropoulos G, Gazouli M, Ikonomopoulos JA, Kantzoura V, Kominakis A (2006) Determination of prevalence and risk factors of infection with Babesia in small ruminants from Greece by polymerase chain reaction amplification. Vet Parasitol 135:99–104
Uilenberg G (2006) Babesia: a historical overview. Vet Parasitol 138(1–2):3–10
Zaeemi M, Haddadzadeh H, Khazraiinia P, Kazemi B, Bandehpour M (2011) Identification of different Theileria species (Theileri lestoquardi, Theileriaovis, and Theileria annulata) in naturally infected sheep using nested PCR-RFL. Parasitol Res 108:837–843
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Sharifi, N., Ganjali, M., Nabavi, R. et al. A study on prevalence and identification of Ovine Theileria and Babesia infection in Zabol using PCR method. J Parasit Dis 40, 1535–1539 (2016). https://doi.org/10.1007/s12639-015-0722-9
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DOI: https://doi.org/10.1007/s12639-015-0722-9