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Evaluation of different Haemonchus contortus antigens for diagnosis of sheep haemonchosis by ELISA and their cross reactivity with other helminthes

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Abstract

Haemonchus contortus (H. contortus) remains important nematode that infecting sheep all over the world. Truthful diagnosis of haemonchosis needs reliable Enzyme linked immune sorbent assay test as well as the immuno-reactive protein profile of different prepared H. contortus antigens; larval (L), excretory secretory product (ESP) and adult somatic H. contortus (AS). The current study fulfilled that L antigen is the talented antigen for such serological diagnosis. Immunodominant band at molecular weight 57 kDa were answerable for highest specificity and accuracy of positive predictive value of this antigen. Moreover, the highest apparent prevalence value was 92 and 75% obtained by L and ESP antigens, respectively. The results of cross reactivity among AS, Monezia expansa (M. expansa) and Fasciola spp. revealed that AS antigen appeared major cross reactivity with other cestode and trematode. Best dilution of serum was (1:800) to rise above this phenomenon.

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Acknowledgements

This work was financially supported by co-project between Egypt and Bulgaria, which entitled ‘‘ Recent trails for diagnosis of haemonchosis’’ and funded from academy of scientific research in Egypt.

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Authors and Affiliations

  1. Department of Parasitology and Animal Diseases, National Research Centre, El- Bohouth Street, Dokki, Post Box 12622, Giza, Egypt

    Omnia M. Kandil, Seham H. M. Hendawy & Amira H. El Namaky

  2. Department of Experimental Parasitology, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev St. Bl.25, 1113, Sofia, Bulgaria

    Margarita P. Gabrashanska & Veselin N. Nanev

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  1. Omnia M. Kandil
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  2. Seham H. M. Hendawy
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  3. Amira H. El Namaky
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  4. Margarita P. Gabrashanska
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  5. Veselin N. Nanev
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Correspondence to Omnia M. Kandil.

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Kandil, O.M., Hendawy, S.H.M., El Namaky, A.H. et al. Evaluation of different Haemonchus contortus antigens for diagnosis of sheep haemonchosis by ELISA and their cross reactivity with other helminthes. J Parasit Dis 41, 678–683 (2017). https://doi.org/10.1007/s12639-016-0865-3

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  • DOI: https://doi.org/10.1007/s12639-016-0865-3

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