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Antioxidant and Antitumoral Properties of Aqueous Fractions from Frozen Sargassum muticum

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Abstract

A novel multistage water-based process was evaluated for the production of sap and other liquid fractions from frozen Sargassum muticum in an attempt to propose an integral and yearly-around utilization of this invasive alga. Three aqueous extracts including (i) the defrosting liquid phase, (ii) the sap liquid obtained by pressing and (iii) the autohydrolysis extract from the solid residue remaining after pressing were characterized for yield, phenolic content, reducing power and antiradical properties. Alginate precipitation and a further liquid–liquid extraction stage using ethyl acetate were assayed to concentrate the phenolic fraction. Alginate exhibited similar viscoelastic features to those commercial available. The aqueous extracts contained 3–12 g phloroglucinol equivalents (PGE)/100 g extract, whereas the ethyl acetate extracts contained up to 55 g PGE/100 g extract and 1 g was as potent ABTS+ radical scavenger as 0.5 g Trolox. The aqueous phase produced by autohydrolysis of the solids were cytotoxic against HCT 116 cells (IC50 < 50 µg mL) without noxious effect on normal cells.

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Acknowledgements

The authors are grateful to the Ministry of Science and Innovation of Spain for the support of the research projects CTM2012-38095 and CTM2015-68503-R, and to the Xunta de Galicia (Centro singular de investigación de Galicia accreditation 2016–2019) and the European Union (European Regional Development Fund - ERDF). Both projects were partially funded by the FEDER Program of the European Union (“Unha maneira de facer Europa”). M.D.T. thanks Spanish Ministry of Economy and Competitiveness for her postdoctoral Grant (IJCI-2016-27535).

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Pérez-Larrán, P., Balboa, E.M., Torres, M.D. et al. Antioxidant and Antitumoral Properties of Aqueous Fractions from Frozen Sargassum muticum. Waste Biomass Valor 11, 1261–1269 (2020). https://doi.org/10.1007/s12649-018-0456-x

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