Skip to main content

Advertisement

SpringerLink
Log in

Ultrasonic bacterial treatment of mineral waters: a study on S. epidermidis, S. warneri, P. aeruginosa and P. mirabilis

  • Thematic Issue
  • Published:
Environmental Earth Sciences Aims and scope Submit manuscript

Abstract

The objective of this work is to evaluate the effects of ultrasound waves on the viability of bacteria that usually occur in mineral waters such as spa thermal waters. The study was carried out at a constant temperature of 309.65 K on two Gram-positive bacteria (Staphylococcus epidermidis, Staphylococcus warneri) and two Gram-negative bacteria (Pseudomonas aeruginosa, Proteus mirabilis) with a concentration of 106 CFU mL−1. A commercial ultrasonic bath was used. The effects of the ultrasounds were analysed using a microcalorimetric technique. The microcalorimetric bacterial growth curves were obtained and, stemming from said curves, the heat released by microorganisms during a culture time of 24 h was calculated. The values of the heat released in the samples treated and untreated with ultrasounds were compared. The ultrasound treatment appears to be effective in inhibiting the growth of the two Gram-positive bacteria, but not in the case of the Gram-negative, which presented greater resistance at the same experimental conditions. This study also shows microcalorimetry as an efficient technique to determine the effect of ultrasound waves on bacterial growth.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Ahmed FIK, Russell C (1975) Synergism between ultrasonic waves and hydrogen peroxide in the killing of micro-organisms. J Appl Bacteriol 39(1):31–40

    Article  Google Scholar 

  • Alliger H (1975) Ultrasonic disruption. Am Lab 10:75–85

    Google Scholar 

  • Calvet E, Prat H (1956) Microcalorimétrie: applications physico-chimiques et biologiques. Masson et Cie Editeurs, Paris

    Google Scholar 

  • Cameron M, McMaster LD, Britz TJ (2008) Electron microscopic analysis of dairy microbes inactivated by ultrasound. Ultrason Sonochem 15(6):960–964

    Article  Google Scholar 

  • Chunmei D, Jiabo W, Weijun K, Cheng P, Xiaohe X (2010) Investigation of anti-microbial activity of catechin on Escherichia coli growth by microcalorimetry. Environ Toxicol Pharmacol 30(3):284–288

    Article  Google Scholar 

  • Drakopoulou S, Terzakis S, Fountoulakis MS, Mantzavinos D, Manios T (2009) Ultrasound-induced inactivation of gram-negative and gram-positive bacteria in secondary treated municipal wastewater. Ultrason Sonochem 16(5):629–634

    Article  Google Scholar 

  • Gao S, Lewis GD, Ashokkumar M, Hemar Y (2014) Inactivation of microorganisms by low-frequency high-power ultrasound: 1. Effect of growth phase and capsule properties of the bacteria. Ultrason Sonochem 21(1):446–453

    Article  Google Scholar 

  • Gómez-López MD, Bayo J, García-Cascales MS, Angosto JM (2009) Decision support in disinfection technologies for treated wastewater reuse. J Clean Prod 17(16):1504–1511

    Article  Google Scholar 

  • James AM (1987) Calorimetry. Past, present and future in thermal and energetic studies of cellular biological systems. IOP Publishing Ltd, Bristol

  • Joyce E, Phull SS, Lorimer JP, Mason TJ (2003) The development and evaluation of ultrasound for the treatment of bacterial suspensions. A study of frequency, power and sonication time on cultured Bacillus species. Ultrason Sonochem 10(6):315–318

    Article  Google Scholar 

  • Lago N, Legido JL, Paz Andrade MI, Arias I, Casás LM (2011) Microcalorimetric study on the growth and metabolism of Pseudomonas aeruginosa. J Therm Anal Calorim 105(2):651–655

    Article  Google Scholar 

  • Leighton TG (1994) The Acoustic Bubble. Academic Press, San Diego

    Google Scholar 

  • Ma J, Qi WT, Yang LN, Yu WT, Xie YB, Wang W, Ma XJ, Xu F, Sun LX (2007) Microcalorimetric study on the growth and metabolism of microencapsulated microbial cell culture. J Microbiol Method 68(1):172–177

    Article  Google Scholar 

  • Maraver F, Armijo F (2010) Vademecum II de aguas mineromedicinales españolas. Editorial Complutense, Madrid

    Google Scholar 

  • Monsen T, Lövgren E, Widerström M, Wallinder L (2009) In vitro effect of ultrasound on bacteria and suggested protocol for sonication and diagnosis of prosthetic infections. J Clin Microbiol 47(8):2496–2501

    Article  Google Scholar 

  • Nesaratnam ST, John Wase DAJ, Blakebrough N (1982) The susceptibility to ultrasonic disintegration of Klebsiella pneumonia NCTC 418. Eur J Appl Microbiol Biotechnol 15(1):56–58

    Article  Google Scholar 

  • Olvera M, Eguía A, Rodríguez O, Chong E, Pillai SD, Ilangovan K (2008) Inactivation of Cryptosporidium parvum oocysts in water using ultrasonic treatment. Bioresour Technol 99(6):2046–2049

    Article  Google Scholar 

  • Paleologou A, Marakas H, Xekoukoulotakis NP, Moya A, Vergara Y, Kalogerakis N, Gikas P, Mantzavinos D (2007) Disinfection of water and wastewater by TiO2 photocatalysis, sonolysis and UV-C irradiation. Catal Today 129:136–142

    Article  Google Scholar 

  • Patil MN, Pandit AB (2007) Cavitation - A novel technique for making stable nano-suspensions. Ultrason Sonochem 14(5):519–530

    Article  Google Scholar 

  • Paz Andrade MI (1967) Les développements récents de la microcalorimétrie et de la thermogenèse, 1st edn. CRNS, Paris

    Google Scholar 

  • Rivero NL, Legido JL, Santos IA, Casás LM (2012) Comparative study of microcalorimetric behavior of Escherichia coli, Proteus mirabilis and Klebsiella pneumoniae. Pol J Microbiol 61(3):199–204

    Google Scholar 

  • Rivero NL, Soto JLL, Santos IA, Casás LM (2013) Differentiation between Staphylococcus aureus and Staphylococcus epidermidis using microcalorimetry. Int J Thermophys 34(6):1039–1048

    Article  Google Scholar 

  • Scherba G, Weigel RM, O’Brien WD (1991) Quantitative assessment of the germicidal efficacy of ultrasonic energy. Appl Environ Microbiol 57(7):2079–2084

    Google Scholar 

  • Shah YT, Pandit AB, Moholkar VS (1999) Cavitation reaction engineering. Plenum Publishers, New York

    Book  Google Scholar 

  • Singer AJ, Coby CT, Singer AH, Thode HC, Tortora GT (1999) The effects of low-frequency ultrasound on Staphylococcus epidermidis. Curr Microbiol 38(3):194–196

    Article  Google Scholar 

  • Thacker J (1973) An approach to the mechanism of killing of cells in suspension by ultrasound. Biochim Biophys Acta 304(2):240–248

    Article  Google Scholar 

  • Villamiel M, De Jong P (2000) Inactivation of Pseudomonas fluorescens and Streptococcus thermophilus in Trypticase Soy Broth and total bacteria in milk by continuous-flow ultrasonic treatment and conventional heating. J Food Eng 45(3):171–179

    Article  Google Scholar 

Download references

Acknowledgments

We thank María Perfecta Salgado González and Sofía Baz Rodríguez for their collaboration with the technical measures. We are also thankful for the financial support provided by the projects EM 2012/141, C269 131H 64502, CN 2012/285, and “Agrupación estratéxica de Biomedicina (INBIOMED)” by “Xunta de Galicia” and the project FIS 2011-23322 funded by Ministry of Science and Innovation of Spain. All these projects are co-financed with FEDER funds.

Author information

Authors and Affiliations

  1. Department of Applied Physics, University of Vigo, Lagoas Marcosende, 36310, Vigo, Spain

    C. Vázquez, T. P. Iglesias, L. Mourelle, C. P. Gómez, M. M. Mato & J. L. Legido

  2. Vigo University Hospital, Pharmacy Service, Pizarro nº 22, 30202, Vigo, Spain

    N. Lago

Authors
  1. C. Vázquez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. P. Iglesias
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. Mourelle
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. P. Gómez
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. N. Lago
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. M. Mato
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J. L. Legido
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C. Vázquez.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Vázquez, C., Iglesias, T.P., Mourelle, L. et al. Ultrasonic bacterial treatment of mineral waters: a study on S. epidermidis, S. warneri, P. aeruginosa and P. mirabilis . Environ Earth Sci 73, 2863–2868 (2015). https://doi.org/10.1007/s12665-014-3488-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12665-014-3488-y

Keywords

Search

Navigation