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Comparative transcriptomic analysis of Listeria monocytogenes reveals upregulation of stress genes and downregulation of virulence genes in response to essential oil extracted from Baccharis psiadioides

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Abstract

Listeria monocytogenes is a pathogenic microorganism in humans and is frequently transmitted by food. Methods to control the presence of Listeria in foods are necessary. In the present study, transcriptomics of L. monocytogenes grown in the presence of essential oil extracted from Baccharis psiadioides were studied by RNA sequencing and reverse transcription quantitative polymerase chain reaction (RT-qPCR) experiments. The results obtained indicate that essential oil of B. psiadioides has potential bacteriostatic activity at the concentration tested, affecting Listeria cells functioning and development. Responses of the microorganism included upregulation of stress genes and downregulation of virulence genes, such as actA, hly and prfA, indicating a decrease in virulence and in the capacity of the microorganism to cause infection. Thus, the results presented here allow us to conclude that B. psiadioides essential oil may be an alternative means of controlling microorganisms proliferating in foods.

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Acknowledgements

We acknowledge the National Council for Scientific and Technological Development of Brazil (CNPq) (J. F. Grants #473181/2013-4 and #303603/2015-1).

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Authors and Affiliations

  1. Postgraduate Program in Food Science and Technology, Food Science and Technology Institute (ICTA), Federal University of Rio Grande do Sul (UFRGS), Bento Gonçalves Ave. 9500 / Building, 43212, Porto Alegre, Rio Grande do Sul (RS), Brazil

    Luiza Pieta, Kamila Patikowski Cheiran & Jeverson Frazzon

  2. Postgraduate Program in Cellular and Molecular Biology, Biotechnology Center (CBiot), Federal University of Rio Grande do Sul (UFRGS), Bento Gonçalves Ave. 9500 / Building, Porto Alegre, RS, 43431, Brazil

    Frank Lino Guzman Escudero & Rogério Margis

  3. Institute for Research in Bioenergy, São Paulo State University (UNESP), 10th St. 2527, Rio Claro, São Paulo (SP), Brazil

    Ana Paula Jacobus & Jeferson Gross

  4. Postgraduate Program in Genetics and Molecular Biology, Federal University of Rio Grande do Sul (UFRGS), Bento Gonçalves Ave. 9500 / Building 43323M, Porto Alegre, RS, Brazil

    Maria Lisseth Eguiluz Moya & Rogério Margis

  5. Department of Botany, Biosciences Institute , Federal University of Rio Grande do Sul (UFRGS), Bento Gonçalves Ave. 9500 / Building, 43433, Porto Alegre, RS, Brazil

    Geraldo Luiz Gonçalves Soares

  6. Department of Microbiology, Immunology and Parasitology, Basic Health Sciences Institute (ICBS), Federal University of Rio Grande do Sul (UFRGS), Sarmento Leite St, Porto Alegre, RS, 500, Brazil

    Ana Paula Guedes Frazzon

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  1. Luiza Pieta
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Pieta, L., Escudero, F.L.G., Jacobus, A.P. et al. Comparative transcriptomic analysis of Listeria monocytogenes reveals upregulation of stress genes and downregulation of virulence genes in response to essential oil extracted from Baccharis psiadioides . Ann Microbiol 67, 479–490 (2017). https://doi.org/10.1007/s13213-017-1277-z

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