Elsevier

Brain Research

Volume 656, Issue 1, 5 September 1994, Pages 169-173
Brain Research

The increase in striatal neuropeptide Y immunoreactivity induced by neonatal dopamine-depleting lesions in rats is reversed by intrastriatal dopamine-rich transplants

https://doi.org/10.1016/0006-8993(94)91379-XGet rights and content

Abstract

The aim of the present experiment was to test whether: (i) the destruction of the dopaminergic meso-telencephalic pathway in neonatal rats induces an increase in the density of Neuropeptide Y immunoreactive (NPY-IR) neuronal perikarya within the denervated neostriatum; (ii) embryonic dopaminergic neurones grafted into the neonatal neostriatum could block such an effect of the lesion. As a control, density of NPY-IR neurones was also examined in rats lesioned and/or grafted at adulthood. The ascending dopaminergic system of 3-day-old rat pups or adult rats was unilaterally lesioned by intrahypothalamic injection of 6-hydroxydopamine. Grafting was performed six days later. The neonatal lesion increased the number of NPY-IR neurones on the lesioned side by 24% as compared to the contralateral neostriatum. This increase was abolished in the neostriatum bearing dopaminergic grafts as evaluated six weeks after grafting. These effects are similar to that observed in animals lesioned and/or grafted as adults and further extend the range of post-lesion modifications which can be reversed by the implantation of embryonic DA neurones to neonates.

References (28)

  • WeihmullerF.B. et al.

    Age-dependent plasticity in the dopaminergic control of sensorimotor development

    Behav. Brain Res.

    (1989)
  • AbrousN. et al.

    Development of intracerebral dopaminergic grafts: a combined immunohistochemical and autoradiographic study of its time course and environmental influences

    J. Comp. Neurol.

    (1988)
  • AbrousD.N. et al.

    Intrastriatal transplants of embryonic dopaminergic neurons counteract the increase of striatal enkephalin immunostaining but not serotoninergic sprouting elicited by a neonatal lesion of the nigrostriatal dopaminergic pathway

    Eur. J. Neurosci.

    (1993)
  • BjörklundA.

    Dopaminergic transplants in experimental parkinsonism: cellular mechanisms of graft-induced recovery

    Curr. Opin. Neurobiol.

    (1992)
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      Upon exposure to l-DOPA, substance P is decreased further in the neonate-lesioned animals, whereas the elevation of enkephalin is further increased [143]. While a unilateral lesion to dopaminergic neurons in neonate rats increases striatal neuropeptide Y, a similar increase is also observed in rats given this lesion as adults [1]. The level of this peptide in the bilaterally neonate-lesioned rats has not been determined.

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