Elsevier

Clinica Chimica Acta

Volume 74, Issue 3, 1 February 1977, Pages 217-225
Clinica Chimica Acta

Factors influencing lysozyme determinations by the lysoplate method

https://doi.org/10.1016/0009-8981(77)90288-1Get rights and content

Abstract

Some factors influencing the lysoplate method have been investigated. The influences of pH, ionic strength and temperature of the gel medium can be explained by considering the influence of these factors upon the diffusion rate rather than upon the enzymatic properties of lysozyme. Salts and proteins present in a sample probably affect the results in a similar way. Although the method has a variation coefficient around 10% and thus an acceptable precision, its accuracy is variable and doubtful. The method could be clinically interesting, by discriminating lysozyme isoenzymes, or by indirectly detecting proteins associated with some disease states.

References (20)

  • K.R. Falchuk et al.

    Gastroenterology

    (1975)
  • A. Schussheim et al.

    Gastroeneterology

    (1976)
  • R.E. Pounder et al.

    Lancet

    (1975)
  • K.S. Isselbacher

    Gastroenterology

    (1976)
  • G. Gorin et al.

    Anal. Biochem.

    (1971)
  • P. Jolles et al.
  • R.E. Canfield et al.
  • R. Trautman

    Biophys. J.

    (1972)
  • B. Goldstein et al.

    J. Theor. Biol.

    (1975)
  • S. Zucker et al.

    J. Lab. Clin. Med.

    (1970)
There are more references available in the full text version of this article.

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    Larvae topically-treated with 2 μL acetone followed by 2 μL saline injection were used as the control. Lysozyme concentration was measured as per Peeters and Vantrappen (1977). A standard curve was prepared between lysozyme concentration and diameter of the clear zones (mm) by serially diluting a 1 mg/mL stock solution of hen egg white lysozyme (HEWL) (Sigma-Aldrich).

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