Elsevier

Life Sciences

Volume 55, Issue 13, 1994, Pages 1027-1032
Life Sciences

Bone marrow oxygen consumption and erythropoiesis in chronically hypoxic rats

https://doi.org/10.1016/0024-3205(94)00637-7Get rights and content

Abstract

Bone marrow oxygen consumption (VO2) was determined weekly in 16 Holtzman rats exposed to continuous hypobaric hypoxia (CHH) during 30 days. The results were compared with those obtained in 10 sea level control animals (SL). The VO2 expressed as ng.at.O2/min, decreased progressively with time of exposure to hypoxia. VO2 (mean±SD) was 0.0936 ± 0.0135 in SL rats. In CHH animals, it was 0.1001 ± 0.0292 after 8 days of hypoxia, 0.1030 ± 0.0206 after 16 days, 0.0594 ± 0.0148(p=0.002) after 24 days and 0.0136 ± 0.0404 (p=0.000) after 30 days. Protein concentration in bone marrow was progressively higher in hypoxics when compared to control, with significant differences since the first week of exposure. Blood hemoglobin increased in parallel to protein concentration in the bone marrow. These findings suggest an increase in the cells of the erythroid series whose oxygen consumption is less than cells in the early stages of differentiation. The increased protein concentration is in agreement with the fact that globin mRNA appears in cells with a progressively increasing anaerobic metabolism at relatively late stages of erythropoiesis.

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Cited by (5)

  • Oxidative stress status in rats after intermittent exposure to hypobaric hypoxia

    2010, Wilderness and Environmental Medicine
    Citation Excerpt :

    After the exposure period was over, the elevated values of RBC, Hb, and Hc tended to return to the lower, normal range. Consequently, it seems clear that intermittent exposure to hypoxia can also stimulate erythropoiesis in the rat to the same extent as chronic exposure.50–52 Slight differences in hematologic indexes may be due to the influence of a significantly higher percentage of reticulocytes in group H rats, which would cause a small increase in mean erythrocyte volume.8,53

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