Characterization of Ca2+ signals generated by extracellular nucleotides in supporting cells of the organ of Corti
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Cited by (80)
Targeted single-cell electroporation loading of Ca<sup>2+</sup> indicators in the mature hemicochlea preparation
2019, Hearing ResearchCitation Excerpt :The preparation was first used by our group for real functional imaging of intracellular Ca2+ signaling, which is implicated in the aforementioned phenomena (Horváth et al., 2016). In that study, the indicator dye was bulk loaded in its AM form, as in the majority of Ca2+ imaging studies on cells in the cochlea (Chan and Rouse, 2016; Dulon et al., 1993; Matsunobu and Schacht, 2000; Piazza et al., 2007). Bulk loading is convenient, but the dye remains in the extracellular space resulting in significant background staining and low S/N. AM dyes can be taken up by every cell, contaminating the responses of the cell of interest by fluorescence from adjacent responding cells (Fridberger et al., 1998).
Ca<sup>2+</sup> signaling, apoptosis and autophagy in the developing cochlea: Milestones to hearing acquisition
2018, Cell CalciumCitation Excerpt :Ca2+ signaling in both sensory and non-sensory cells is emerging as a crucial component of the complex and incompletely defined morphogenetic scenario that leads to hearing acquisition [83–85]. In the developing organ of Corti, extracellular adenosine trisphosphate (ATP), a paracrine factor that is key to cochlear physiology and pathology [86–97], triggers oscillations of the cytosolic free calcium concentration ([Ca2+]c) and propagation of intercellular Ca2+ waves [98–101] (Video S3). Connexin hemichannels [102] expressed at the endolymphatic surface of cochlear non-sensory cells [103,104] (Fig. 4) play a crucial role in this scenario, as: (I) ATP binding to P2Y receptors activates phospholipase C-dependent IP3 production, which (II) promotes Ca2+ release from the endoplasmic reticulum (ER) (III) raising the [Ca2+]c. (IV) Up to ∼500 nM, the [Ca2+]c increases the hemichannel open probability (V) fostering ATP release from cytoplasm to endolymph through connexin hemichannels (Video S4).
Purinergic signaling in special senses
2009, Trends in NeurosciencesPostnatal development of the organ of Corti in dominant-negative Gjb2 transgenic mice
2008, NeuroscienceCitation Excerpt :ATP is known to act as trophic factor, mitogen and potent neuromodulator (Fields and Burnstock, 2006; Nedergaard et al., 2003). Since supporting cells express ATP receptors (Dulon et al., 1993), the same scenario is likely to occur in an adapted form with the organ of Corti. The supporting cells may have an influence on maturation, cell volume and cell shape at least through the polymerization of microtubules by activated by ATP in an autocrine and paracrine manner (Zhao et al., 2005).
Supporting Cells–a New Area in Cochlear Physiology Study
2008, Journal of OtologyCitation Excerpt :Identified as a neuromodulater or neurotransmitter2, 19, 20, ATP exerts its effects mainly through P2 purinergic receptors. Studies showed that ATP can induce Potassium current on Hensen’s cells 21, elevate the concentration of free calcium in the inner and outer hair cells 5, 22–26, and also stimulate Ca2 + release from internal stores in Deiters’ cells (but not in Hensen’s cells27). ATP mobilized Ca2 + from the phalanges of Deiters’ cells, which then propagated towards the cell body as a wave, suggestisng presence of purinergic receptors in the phalanges.