Neuron
Volume 8, Issue 1, January 1992, Pages 135-143
Journal home page for Neuron

Article
Calcium influx through nicotinic receptor in rat central neurons: Its relevance to cellular regulation

https://doi.org/10.1016/0896-6273(92)90115-TGet rights and content

Abstract

The Ca2+ permeability of a nicotinic acetylcholine receptor (nAChR) in the rat CNS was determined using both current and fluorescence measurements on medial habenula neurons. The elementary slope conductance of the nAChR channel was 11 pS in pure external Ca2+ (100 mM) and 42 pS in standard solution. Ca2+ influx through nAChRs resulted in the rise of cytosolic Ca2+ concentration ([Ca2+]i) to the micromolar range. This increase was maximal under voltage conditions (below −50 mV) in which Ca2+ influx through voltage-activated channels was minimal. Ca2+ influx through nAChRs directly activated a Ca2+-dependent Cl conductance. In addition, it caused a decrease in the GABAA response that outlasted the rise in [Ca2+]i. These results underscore the physiological significance of Ca2+ influx through nAChR channel in the CNS.

References (41)

  • E.R. Decker et al.

    Calcium permeability of the nicotinic acetylcholine receptor: the single-channel calcium influx is significant

    J. Neurosci.

    (1990)
  • E.M. DeLorme et al.

    Effect of prolonged depolarization on the nicotinic acetylcholine receptor of PC12 cells

    J. Neurochem.

    (1988)
  • K. Fuxe et al.

    On the role of receptor-receptor interactions in synaptic transmission: biochemical and autoradiographic studies on the interactions between α2-adrenergic and neuropeptide Y receptors in the nucleus tractus solitarius

  • J.-L. Galzi et al.

    Functional architecture of the nicotinic acetylcholine receptor: from the electric organ to brain

    Annu. Rev. Pharmacol.

    (1991)
  • T.A. Gilbertson et al.

    Permeation of calcium through non-NMDA glutamate channels in retinal bipolar cells

    Science

    (1991)
  • M. Hollmann et al.

    Ca2+ permeability of KA-AMPA-gated glutamate receptor channels depends on subunit composition

    Science

    (1991)
  • R.W. Holz et al.

    Relationship between Ca2+ uptake and catecholamine secretion in primary dissociated cultures of adrenal medulla

    J. Neurochem.

    (1982)
  • M. Iino et al.

    Permeation through excitatory amino acid receptor channels in cultured rat hippocampal neurones

    J. Physiol.

    (1990)
  • M. Inoue et al.

    Intracellular calcium ions decrease the affinity of the GABA receptor

    Nature

    (1986)
  • F. Jaramillo et al.

    Embryonic acetylcholine receptors guarantee spontaneous contractions in rat developing muscle

    Nature

    (1988)
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