Original contributionA comparative study of preparation techniques for improving the viability of nigral grafts using vital stains, in vitro cultures, and in vivo grafts
References (68)
Parkinson's disease: Pathophysiology
Lancet
(1991)Cerebral transplantation for Parkinson's disease: Current progress and future prospects
- et al.
A functional assessment of embryonic dopaminergic grafts in the marmoset
Prog. Brain Res.
(1990) - et al.
Neurotrophic factors and neural grafts: A growing field
Sem. Neurosci.
(1993) - et al.
Factors important in the survival of dopamine neurons in intracerebral grafts of embryonic substantia nigra
Dopaminergic transplants in experimental parkinsonism: Cellular mechanisms of graft induced functional recovery
Curr. Opin. Neurobiol.
(1992)- et al.
Monitoring of cell viability in suspensions of embryonic CNS tissue and its use as a criterion for intracerebral graft survival
Brain Res
(1985) - et al.
Survival of intracerebrally grafted rat dopamine neu rones previously cultured in vitro
Neurosci. Lett.
(1985) - et al.
Survival and function of dissociated rat dopamine neurones grafted at different neural stages or after being cultured in vitro
Dev. Brain Res.
(1988) - et al.
Co-grafts of embryonic dopamine neurons and adult sciatic nerve into the denervated striatum enhance behavioral and morphological recovery in rats
Exp. Neurol.
(1991)
Sequential intrastriatal grafting of allogeneic embryonic dopamine-rich tissue in adult rats:Will the second graft be rejected?
Neurosci
Grafts of embryonic substantia nigra reinnervating the ventrolateral striatum ameliorate sensorimotor impairments and akinesia in rats with 6-OHDA lesions of the nigrostriatal pathway
Brain Res
Dopaminerich transplants in experimental parkinsonism
Trends. Neurosci.
Dopamine-rich grafts ameliorate whole body motor asymmetry and sensory neglect but not independent limb use in rats with 6-hydroxyamine lesions
Brain Res.
Basic fibroblast growth factor promotes the survival and development of mesencephalic neurons in culture
Dev. Biol.
Nimodipine enhances growth and vascularisation of neural grafts
Exp. Neurol.
A comparison of solid intraventricular and dissociated intraparenchymal fetal substantia nigra grafts in rat model of Parkinson's disease: Impaired graft survival is associated with high baseline rotational behavior
Exp. Neurol.
Reinnervation of the nucleus accumbens and frontal cortex of the rat by dopaminer gic grafts and effects on hoarding behaviour
Brain Res.
Naturally occurring cell death during postnatal development of the substantia nigra pars compacta of rat
Mol. Cell. Neurosci.
Cryopreservation of primary neurons for tissue culture
Brain Res.
Prospects of transplantation in human neurodegenerative diseases
Trends Neurosci.
Basic FGF promotes the survival of embryonic ventral mesencephalic dopaminergic neurons. 1. Effects in vitro
Neurosci.
Basic FGF promotes the survival of embryonic ventral mesencephalic dopaminergic neurones. II. Effects on nigral transplants in vivo
Neurosci.
Improved graft survival and striatal reinnervation by microtransplantation of fetal nigral cell suspensions in the rat Parkinson model
Brain Res.
L1 substrate enhances outgrowth of tyrosine hydroxylase immunoreactive neurites in mesencephalic cell cultures
Exp. Neurol.
Dopaminergic neuron development in rats: Biochemical study from prenatal life to adulthood
Brain Res. Bull.
Brain derived neurotrophic factor enhances function rather than survival of intrastriatal dopamine cell-rich grafts
Brain Res.
Intracerebral grafting of dissociated CNS tissue suspensions
Mesencephalic dopaminergic cells exhibit increased density of neural cell adhesion molecule and polysialic acid during development
Dev. Brain Res.
Fetal dopamine cell survival after transplantation is dramatically improved at a critical donor gestational age in nonhuman primates
Exp. Neurol.
Postnatal changes in the distribution and morphology of rat substantia nigra neurons
Neurosci.
Quantitative recording of rotational behaviour in rats with 6-hydroxy-dopamine lesions of the nigro-striatal dopamine systems
Brain Res.
The pre and postnatal development of the dopaminergic cell groups in the ventral mesen cephalon and the dopaminergic innervation of the striatum of the rat
Neurosci.
Estimation of the nuclear population from microtome sections
Anat. Rec.
Cited by (48)
Survival, differentiation, and connectivity of ventral mesencephalic dopamine neurons following transplantation
2012, Progress in Brain ResearchCitation Excerpt :Gradual improvements on various aspects related to tissue handling prior to implantation have also greatly enhanced the yield of DA neurons from VM pieces after grafting. While optimization of parameters such as dissection technique and choice of media have clearly been important (Barker et al., 1995; Brundin et al., 1985b, 2000), one of the most fundamental changes to the preparation of VM tissue for transplantation has been the development of the cell suspension technique, whereby the solid VM piece is dissociated into a roughly single cell suspension through incubation with trypsin (and DNase) followed by mechanical dissociation (Björklund et al., 1983a,b). The approach has important practical advantages over solid VM piece grafts, including the possibility to consistently deliver deposits of predefined cell numbers across multiple graft sites and animals.
Nigral grafts in animal models of Parkinson's disease. Is recovery beyond motor function possible?
2012, Progress in Brain ResearchCitation Excerpt :Transplantation of small pieces of embryonic nigral tissue into the cortex or ventricle of unilateral-lesioned rats was seen to alleviate motor asymmetries induced by unilateral DA-system damage in simple quantitative tests of spontaneous, amphetamine- and apomorphine-induced rotation (Björklund and Stenevi, 1979; Björklund et al., 1980a,b; Perlow et al., 1979), and these original observations have been replicated in many hundreds of experiments using more modern and flexible cell transplantation procedures (Björklund et al., 1980b; Dunnett and Björklund, 2010; Dunnett et al., 1988). In the intervening three decades, the nigral transplantation model has provided the bedrock for systematic experimental analysis of the practical protocols to achieve reliable, effective, and efficient engraftment of the transplanted cells (Barker et al., 1995); to elucidate the mechanisms of integration and functional recovery at anatomical, physiological, and behavioral levels (Björklund et al., 1987; Dunnett and Björklund, 2010); and nowadays to provide the foremost platform for developing stem cell therapies in the central nervous system for neurological disease (Arenas, 2010; Lindvall and Kokaia, 2010). On the basis of such studies, PD has provided over the past 25 years the principle target for “first-in-man” trials translating experimental transplantation procedures into the clinic.
Characterization of axon guidance cue sensitivity of human embryonic stem cell-derived dopaminergic neurons
2010, Molecular and Cellular NeuroscienceRecovery of functional deficits following early donor age ventral mesencephalic grafts in a rat model of Parkinson's disease
2008, NeuroscienceCitation Excerpt :The range of techniques used is too large for a detailed description here but it is worth mentioning the main areas of interest by contrast to the donor age approach taken in the current paper. In general, enhancement of DA grafts has involved three main strategies: first, there have been improvements in the dissection, preparation and implantation of VM tissue, resulting in more viable, longer lasting cell suspensions, and using less traumatic methods of implanting cells into the brain (Barker et al., 1995; Brundin et al., 1985; Fricker-Gates et al., 2001); second, DA neuron survival has been enhanced by treatment with trophic factors. For example, in rat models of PD, DA cell yields in grafts have been successfully boosted up to 30% using glial derived neurotrophic factor (GDNF) (Rosenblad et al., 1996; Sautter et al., 1998; Sinclair et al., 1996; Sullivan et al., 1998; Yurek, 1998), up to 40% using neurturin (Rosenblad et al., 1999), up to 34% using fibroblast growth factor beta, (bFGF) (Takayama et al., 1995) and up to 18% using growth/differentiation factor five (GDF5) (Sullivan et al., 1998).