Short communication
Antibiotic resistance of Aeromonas hydrophila isolated from marketed fish and prawn of South India

https://doi.org/10.1016/S0168-1605(02)00009-0Get rights and content

Abstract

A total of 319 strains of Aeromonas hydrophila were isolated from 536 fish and 278 prawns for a 2-year period. All the strains were tested for resistance to 15 antibiotics and 100% of the strains was resistant to methicillin and rifampicin followed by bacitracin and novobiocin (99%). Only 3% of the strains exhibited resistance against chloramphenicol. The multiple antibiotic resistance (MAR) indexing of A. hydrophila strains showed that all of them originated from high-risk sources.

Introduction

Wide use of antibiotics to treat bacterial infections and incorporation of subtherapeutic dose of antibiotics into feeds for cultured organism resulted in a global increase in antibiotic resistance among pathogenic bacteria. The problem is more serious in developing countries, where antibiotics are used widely. In India, antibiotics are extensively applied in animal husbandry and aquaculture.

The use of antibiotics is the most important factor in amplifying the level of resistance in a given reservoir (Wegener and Frimodt-Moller, 2000). Multiple antibiotic resistance (MAR) among Aeromonas hydrophila strains has been reported from many parts of the world Pettibone et al., 1996, Son et al., 1997, Ko et al., 1998, Rajeswari Shome and Shome, 1999. Under these circumstances, it will be worthwhile to find out the prevalence of antibiotic resistance of the Aeromonas strains that may be considered as an emerging pathogen and to identify the high-risk source.

Section snippets

Materials and methods

The fish and prawn samples were collected from a major fish market of Coimbatore, Tamil Nadu, South India. The samples were collected in sterile polyethylene bags and brought to the laboratory in an ice chest. The samples were processed within 2 h of collection. Body surface, gill and intestinal content of fish were aseptically swabbed using sterile cotton buds, inoculated into alkaline peptone water (peptone—10.0 g; sodium chloride—10.0 g; distilled water—1000 ml; pH—8.4, Shread et al., 1981)

Results and discussion

The percentage of A. hydrophila strains showing resistance against each antibiotic is given in Table 1. All the strains were resistant to methicillin, which was similar to the findings of Motyl et al. (1985) who reported that all A. hydrophila strains of human origin were resistant to methicillin. In contrast, Pettibone et al. (1996) observed that only 54% of the strains was resistant to this antibiotic. However, Kampfer et al. (1999) reported that no significant differences could be observed

Acknowledgments

The authors are thankful to the Head, Department of Environmental Sciences, Bharathiar University, Coimbatore for providing necessary laboratory facilities and also to Indian Council of Agricultural Research (4 (49)/97-ASR-1 dt 22.09.2001) for the financial support.

References (23)

  • W.C. Ko et al.

    Increasing antibiotic resistance in clinical isolates of Aeromonas strains in Taiwan

    Antimicrob. Agents Chemother.

    (1996)
  • Cited by (0)

    View full text